Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They are very effective at identifying DNA repair pathways, highlighting new damage-dependent roles for the Slt2 kinase, Pph3 phosphatase, and histone variant Htz1. The data also reveal that protein complexes are generally stable in response to perturbation, but the functional relations between these complexes are substantially reorganized. Differential networks chart a new type of genetic landscape that is invaluable for mapping cellular responses to stimuli.
%0 Journal Article
%1 Bandyopadhyay2010Rewiring
%A Bandyopadhyay, Sourav
%A Mehta, Monika
%A Kuo, Dwight
%A Sung, Min-Kyung K.
%A Chuang, Ryan
%A Jaehnig, Eric J.
%A Bodenmiller, Bernd
%A Licon, Katherine
%A Copeland, Wilbert
%A Shales, Michael
%A Fiedler, Dorothea
%A Dutkowski, Janusz
%A Guénolé, Aude
%A van Attikum, Haico
%A Shokat, Kevan M.
%A Kolodner, Richard D.
%A Huh, Won-Ki K.
%A Aebersold, Ruedi
%A Keogh, Michael-Christopher C.
%A Krogan, Nevan J.
%A Ideker, Trey
%D 2010
%I American Association for the Advancement of Science
%J Science (New York, N.Y.)
%K gene-networks
%N 6009
%P 1385--1389
%R 10.1126/science.1195618
%T Rewiring of genetic networks in response to DNA damage.
%U http://dx.doi.org/10.1126/science.1195618
%V 330
%X Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They are very effective at identifying DNA repair pathways, highlighting new damage-dependent roles for the Slt2 kinase, Pph3 phosphatase, and histone variant Htz1. The data also reveal that protein complexes are generally stable in response to perturbation, but the functional relations between these complexes are substantially reorganized. Differential networks chart a new type of genetic landscape that is invaluable for mapping cellular responses to stimuli.
@article{Bandyopadhyay2010Rewiring,
abstract = {Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to {DNA} damage. Differential interactions uncover many gene functions that go undetected in static conditions. They are very effective at identifying {DNA} repair pathways, highlighting new damage-dependent roles for the Slt2 kinase, Pph3 phosphatase, and histone variant Htz1. The data also reveal that protein complexes are generally stable in response to perturbation, but the functional relations between these complexes are substantially reorganized. Differential networks chart a new type of genetic landscape that is invaluable for mapping cellular responses to stimuli.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Bandyopadhyay, Sourav and Mehta, Monika and Kuo, Dwight and Sung, Min-Kyung K. and Chuang, Ryan and Jaehnig, Eric J. and Bodenmiller, Bernd and Licon, Katherine and Copeland, Wilbert and Shales, Michael and Fiedler, Dorothea and Dutkowski, Janusz and Gu\'{e}nol\'{e}, Aude and van Attikum, Haico and Shokat, Kevan M. and Kolodner, Richard D. and Huh, Won-Ki K. and Aebersold, Ruedi and Keogh, Michael-Christopher C. and Krogan, Nevan J. and Ideker, Trey},
biburl = {https://www.bibsonomy.org/bibtex/20dc892cb103b660f43f6213c756e47b5/karthikraman},
citeulike-article-id = {8351302},
citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1195618},
citeulike-linkout-1 = {http://www.sciencemag.org/content/330/6009/1385.abstract},
citeulike-linkout-2 = {http://www.sciencemag.org/content/330/6009/1385.full.pdf},
citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/330/6009/1385},
citeulike-linkout-4 = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3006187/},
citeulike-linkout-5 = {http://view.ncbi.nlm.nih.gov/pubmed/21127252},
citeulike-linkout-6 = {http://www.hubmed.org/display.cgi?uids=21127252},
day = 3,
doi = {10.1126/science.1195618},
interhash = {24e94d94136c019c045a2e0589cf3b14},
intrahash = {0dc892cb103b660f43f6213c756e47b5},
issn = {1095-9203},
journal = {Science (New York, N.Y.)},
keywords = {gene-networks},
month = dec,
number = 6009,
pages = {1385--1389},
pmcid = {PMC3006187},
pmid = {21127252},
posted-at = {2011-02-02 17:13:41},
priority = {2},
publisher = {American Association for the Advancement of Science},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {Rewiring of genetic networks in response to {DNA} damage.},
url = {http://dx.doi.org/10.1126/science.1195618},
volume = 330,
year = 2010
}