Magnitudes and orientations of the 15N chemical shift tensor of 1-15N-2'-deoxyguanosine determined on a polycrystalline sample by two-dimensional solid-state \NMR\ spectroscopy
The magnitudes and orientations of the 15N chemical shift tensor of 1-15N-2'-deoxyguanosine were determined from a polycrystalline sample using the two-dimensional PISEMA experiment. The magnitudes of the principal values of the 15N chemical shift tensor of the N1 nitrogen of 1-15N-2'-deoxyguanosine were found to be sigma11 = 54 ppm, sigma22 = 148 ppm, and sigma33 = 201 ppm with respect to (15NH4)2SO4 in aqueous solution. Comparisons of experimental and simulated two-dimensional powder pattern spectra show that sigma33N is approximately collinear with the N-H bond. The tensor orientation of sigma33N for N1 of 1-15N-2'-deoxyguanosine is similar to the values obtained for the side chain residues of 15Nepsilon1-tryptophan and 15Npi-histidine even though the magnitudes differ significantly.
%0 Journal Article
%1 lorigan_magnitudes_1999
%A Lorigan, G A
%A McNamara, R
%A Jones, R A
%A Opella, S J
%D 1999
%J J. Magn. Reson.
%K Ammonium Bonding,Magnetic Isotopes,Tryptophan Resonance Simulation,Crystallization,Deoxyguanosine,Histidine,Hydrogen,Hydrogen Spectroscopy,Models,Nitrogen,Nitrogen Sulfate,Chemical,Computer
%N 2
%P 315--319
%R 10.1006/jmre.1999.1822
%T Magnitudes and orientations of the 15N chemical shift tensor of 1-15N-2'-deoxyguanosine determined on a polycrystalline sample by two-dimensional solid-state \NMR\ spectroscopy
%V 140
%X The magnitudes and orientations of the 15N chemical shift tensor of 1-15N-2'-deoxyguanosine were determined from a polycrystalline sample using the two-dimensional PISEMA experiment. The magnitudes of the principal values of the 15N chemical shift tensor of the N1 nitrogen of 1-15N-2'-deoxyguanosine were found to be sigma11 = 54 ppm, sigma22 = 148 ppm, and sigma33 = 201 ppm with respect to (15NH4)2SO4 in aqueous solution. Comparisons of experimental and simulated two-dimensional powder pattern spectra show that sigma33N is approximately collinear with the N-H bond. The tensor orientation of sigma33N for N1 of 1-15N-2'-deoxyguanosine is similar to the values obtained for the side chain residues of 15Nepsilon1-tryptophan and 15Npi-histidine even though the magnitudes differ significantly.
@article{lorigan_magnitudes_1999,
abstract = {The magnitudes and orientations of the 15N chemical shift tensor of [1-15N]-2'-deoxyguanosine were determined from a polycrystalline sample using the two-dimensional PISEMA experiment. The magnitudes of the principal values of the 15N chemical shift tensor of the N1 nitrogen of [1-15N]-2'-deoxyguanosine were found to be sigma11 = 54 ppm, sigma22 = 148 ppm, and sigma33 = 201 ppm with respect to (15NH4)2SO4 in aqueous solution. Comparisons of experimental and simulated two-dimensional powder pattern spectra show that sigma33N is approximately collinear with the N-H bond. The tensor orientation of sigma33N for N1 of [1-15N]-2'-deoxyguanosine is similar to the values obtained for the side chain residues of 15Nepsilon1-tryptophan and 15Npi-histidine even though the magnitudes differ significantly.},
added-at = {2017-03-14T02:48:56.000+0100},
author = {Lorigan, G A and McNamara, R and Jones, R A and Opella, S J},
biburl = {https://www.bibsonomy.org/bibtex/210783a8bafcc57fd9c7496b4ebd0e20c/nmrresource},
doi = {10.1006/jmre.1999.1822},
interhash = {d2f925943abf0985c64d99bc632a0a8f},
intrahash = {10783a8bafcc57fd9c7496b4ebd0e20c},
issn = {1090-7807},
journal = {J. Magn. Reson.},
keywords = {Ammonium Bonding,Magnetic Isotopes,Tryptophan Resonance Simulation,Crystallization,Deoxyguanosine,Histidine,Hydrogen,Hydrogen Spectroscopy,Models,Nitrogen,Nitrogen Sulfate,Chemical,Computer},
month = oct,
number = 2,
pages = {315--319},
pmid = {10497038},
timestamp = {2017-03-14T02:49:21.000+0100},
title = {{Magnitudes and orientations of the 15N chemical shift tensor of [1-15N]-2'-deoxyguanosine determined on a polycrystalline sample by two-dimensional solid-state {\{}NMR{\}} spectroscopy}},
volume = 140,
year = 1999
}