%0 Journal Article %1 Figueroa1998 %A Figueroa, J. V. %A Alvarez, J. A. %A Ramos, J. A. %A Rojas, E. E. %A Santiago, C. %A Mosqueda, J. J. %A Vega, C. A. %A Buening, G. M. %D 1998 %J Annals of The New York Academy Of Sciences %K Follow-UpStudies Animals parasitology Babesiosis therapeuticuse epidemiology isolation/&/purification Oxytetracycline Anaplasma Babesiabovis CattleDiseases Cattle Transportation Anaplasmosis Mexico drugtherapy/epidemiology/prevention/&/control PolymeraseChainReaction Babesia Ticks %P 1--10 %T Bovine babesiosis and anaplasmosis follow-up on cattle relocated in an endemic area for hemoparasitic diseases. %V 849 %X A multiplex PCR/DNA probe assay was used to monitor Babesia bovis, B. bigemina and Anaplasma marginale infection in cattle introduced to a Boophilus microplus-infested area in Veracruz, Mexico. Eight intact, 18-month-old, cross-bred beef cattle (four naive, Group A; four Babesia species--premunized, Group B) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (PE) to ticks. Blood sample analysis for DNA detection by the MPCR/DNA probe assay showed that Group A animals were infected with B. bovis from day 11 up to day 22 PE, requiring treatment on days 17-20. Group B animals were detected positive to B. bovis on days 17-20, did not require treatment and remained persistently infected from days 70 to 84 PE. Treatment of Group A animals delayed the infection with B. bigemina. These animals became positive to the parasite on days 63-77 PE. In contrast, Group B animals (untreated) showed B. bigemina infection on days 21-26 and 63-84 PE. One animal was positive for A. marginale infection on days 63-66 PE, the rest of the animals became so on days 80-98 PE. All infected animals required treatment with oxytetracycline. Monitoring the triple hemoparasite infection with the MPCR/DNA probe assay provided important epidemiological information. Thus, precautionary measures can be established when cattle are moved to a babesiosis/anaplasmosis risk area.

@article{Figueroa1998, abstract = {A multiplex PCR/DNA probe assay was used to monitor Babesia bovis, B. bigemina and Anaplasma marginale infection in cattle introduced to a Boophilus microplus-infested area in Veracruz, Mexico. Eight intact, 18-month-old, cross-bred beef cattle (four naive, Group A; four Babesia species--premunized, Group B) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (PE) to ticks. Blood sample analysis for DNA detection by the MPCR/DNA probe assay showed that Group A animals were infected with B. bovis from day 11 up to day 22 PE, requiring treatment on days 17-20. Group B animals were detected positive to B. bovis on days 17-20, did not require treatment and remained persistently infected from days 70 to 84 PE. Treatment of Group A animals delayed the infection with B. bigemina. These animals became positive to the parasite on days 63-77 PE. In contrast, Group B animals (untreated) showed B. bigemina infection on days 21-26 and 63-84 PE. One animal was positive for A. marginale infection on days 63-66 PE, the rest of the animals became so on days 80-98 PE. All infected animals required treatment with oxytetracycline. Monitoring the triple hemoparasite infection with the MPCR/DNA probe assay provided important epidemiological information. Thus, precautionary measures can be established when cattle are moved to a babesiosis/anaplasmosis risk area.}, added-at = {2010-01-14T17:44:17.000+0100}, author = {Figueroa, J. V. and Alvarez, J. A. and Ramos, J. A. and Rojas, E. E. and Santiago, C. and Mosqueda, J. J. and Vega, C. A. and Buening, G. M.}, biburl = {https://www.bibsonomy.org/bibtex/21601c3daf13890533fab9f10351f135f/uvesco}, file = {:Figueroa1998.pdf:PDF}, institution = {CENID-PAVET, INIFAP-SAGAR Jiutepec, Morelos, Mexico.}, interhash = {22a7a00aba01c05c93150b83b8a6e6bd}, intrahash = {1601c3daf13890533fab9f10351f135f}, journal = {Annals of The New York Academy Of Sciences}, keywords = {Follow-UpStudies Animals parasitology Babesiosis therapeuticuse epidemiology isolation/&/purification Oxytetracycline Anaplasma Babesiabovis CattleDiseases Cattle Transportation Anaplasmosis Mexico drugtherapy/epidemiology/prevention/&/control PolymeraseChainReaction Babesia Ticks}, language = {eng}, medline-pst = {ppublish}, month = Jun, pages = {1--10}, pmid = {9668444}, timestamp = {2010-01-14T17:44:18.000+0100}, title = {Bovine babesiosis and anaplasmosis follow-up on cattle relocated in an endemic area for hemoparasitic diseases.}, volume = 849, year = 1998 }