Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis (MLVA) for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis (PFGE). For MLVA, a multiplex PCR assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. MLVA was highly reproducible and provided results within 6 h. Overall, the discriminatory power of MLVA was equivalent to that of PFGE. The utilization of MLVA for subtyping N. meningitidis isolates provides a rapid and safer alternative to PFGE for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis.
%0 Journal Article
%1 killoran_rapid_2006
%A Killoran, Patrick B.
%A O'Connell, Janice
%A Mothershed, Elizabeth A.
%A Probert, Will S.
%D 2006
%J Journal of Microbiological Methods
%K Meningococcal Molecular Tandem disease, repeats subtyping,
%N 2
%P 330--338
%R 10.1016/j.mimet.2006.04.004
%T Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis
%U http://www.sciencedirect.com/science/article/B6T30-4K428GC-2/2/7759e2053e6abc09da28312234fa5ff1
%V 67
%X Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis (MLVA) for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis (PFGE). For MLVA, a multiplex PCR assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. MLVA was highly reproducible and provided results within 6 h. Overall, the discriminatory power of MLVA was equivalent to that of PFGE. The utilization of MLVA for subtyping N. meningitidis isolates provides a rapid and safer alternative to PFGE for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis.
@article{killoran_rapid_2006,
abstract = {Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis {(MLVA)} for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis {(PFGE).} For {MLVA,} a multiplex {PCR} assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. {MLVA} was highly reproducible and provided results within 6 h. Overall, the discriminatory power of {MLVA} was equivalent to that of {PFGE.} The utilization of {MLVA} for subtyping N. meningitidis isolates provides a rapid and safer alternative to {PFGE} for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Killoran, Patrick B. and {O'Connell}, Janice and Mothershed, Elizabeth A. and Probert, Will S.},
biburl = {https://www.bibsonomy.org/bibtex/22bf70e88afec4084bdaf31006ea43fa5/jelias},
doi = {10.1016/j.mimet.2006.04.004},
interhash = {683ffd80d7d9823290f0c2611765ccfa},
intrahash = {2bf70e88afec4084bdaf31006ea43fa5},
issn = {0167-7012},
journal = {Journal of Microbiological Methods},
keywords = {Meningococcal Molecular Tandem disease, repeats subtyping,},
month = nov,
number = 2,
pages = {330--338},
timestamp = {2011-03-11T10:06:40.000+0100},
title = {Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis},
url = {http://www.sciencedirect.com/science/article/B6T30-4K428GC-2/2/7759e2053e6abc09da28312234fa5ff1},
volume = 67,
year = 2006
}