Two systems, the Identicult-Neisseria (IDN; Scott Laboratories, Inc., Fiskeville, R.I.) strip and the Neisseria/Haemophilus Identification Test Kit (NHI; Vitek Systems, Inc., Hazelwood, Mo.) card, were compared with the 4-h Minitek system (BBL Microbiology Systems, Cockeysville, Md.) for their ability to rapidly identify 157 pathogenic Neisseria and Branhamella catarrhalis isolates. IDN, limited in its identification to four species, when incubated at 35 degrees C for 10 min identified 99\% of the isolates. However, when IDN was incubated at 22 degrees C for 20 min, it identified only 92\% of the isolates. The NHI card, a rapid semiautomated system with the ability to identify 25 organisms to the species level, correctly identified all of the isolates. A test for beta-lactamase production included in the NHI card identified the 12 Neisseria gonorrhoeae and 10 B. catarrhalis beta-lactamase-positive isolates included in the study. The IDN strip (35 degrees C) and the NHI card compared favorably with the Minitek system.
%0 Journal Article
%1 hughes_rapid_1987
%A Hughes, B
%A Pezzlo, M T
%A de la Maza, L M
%A Peterson, E M
%D 1987
%J Journal of Clinical Microbiology
%K Diagnostic, Factors Kits, Moraxella Neisseria Neisseria, Reagent Strips, Time catarrhalis, gonorrhoeae, meningitidis, {(Branhamella)}
%N 11
%P 2223--2224
%T Rapid identification of pathogenic Neisseria species and Branhamella catarrhalis
%U http://www.ncbi.nlm.nih.gov/pubmed/3121668
%V 25
%X Two systems, the Identicult-Neisseria (IDN; Scott Laboratories, Inc., Fiskeville, R.I.) strip and the Neisseria/Haemophilus Identification Test Kit (NHI; Vitek Systems, Inc., Hazelwood, Mo.) card, were compared with the 4-h Minitek system (BBL Microbiology Systems, Cockeysville, Md.) for their ability to rapidly identify 157 pathogenic Neisseria and Branhamella catarrhalis isolates. IDN, limited in its identification to four species, when incubated at 35 degrees C for 10 min identified 99\% of the isolates. However, when IDN was incubated at 22 degrees C for 20 min, it identified only 92\% of the isolates. The NHI card, a rapid semiautomated system with the ability to identify 25 organisms to the species level, correctly identified all of the isolates. A test for beta-lactamase production included in the NHI card identified the 12 Neisseria gonorrhoeae and 10 B. catarrhalis beta-lactamase-positive isolates included in the study. The IDN strip (35 degrees C) and the NHI card compared favorably with the Minitek system.
@article{hughes_rapid_1987,
abstract = {Two systems, the {Identicult-Neisseria} {(IDN;} Scott Laboratories, Inc., Fiskeville, {R.I.)} strip and the {Neisseria/Haemophilus} Identification Test Kit {(NHI;} Vitek Systems, Inc., Hazelwood, Mo.) card, were compared with the 4-h Minitek system {(BBL} Microbiology Systems, Cockeysville, Md.) for their ability to rapidly identify 157 pathogenic Neisseria and Branhamella catarrhalis isolates. {IDN,} limited in its identification to four species, when incubated at 35 degrees C for 10 min identified 99\% of the isolates. However, when {IDN} was incubated at 22 degrees C for 20 min, it identified only 92\% of the isolates. The {NHI} card, a rapid semiautomated system with the ability to identify 25 organisms to the species level, correctly identified all of the isolates. A test for beta-lactamase production included in the {NHI} card identified the 12 Neisseria gonorrhoeae and 10 B. catarrhalis beta-lactamase-positive isolates included in the study. The {IDN} strip (35 degrees C) and the {NHI} card compared favorably with the Minitek system.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Hughes, B and Pezzlo, M T and de la Maza, L M and Peterson, E M},
biburl = {https://www.bibsonomy.org/bibtex/22c5396a7184856f5231b0bb63d9c9dbb/jelias},
interhash = {698c76abbe51d96a50dcaf33078930b2},
intrahash = {2c5396a7184856f5231b0bb63d9c9dbb},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
keywords = {Diagnostic, Factors Kits, Moraxella Neisseria Neisseria, Reagent Strips, Time catarrhalis, gonorrhoeae, meningitidis, {(Branhamella)}},
month = nov,
note = {{PMID:} 3121668},
number = 11,
pages = {2223--2224},
timestamp = {2011-03-11T10:06:40.000+0100},
title = {Rapid identification of pathogenic Neisseria species and Branhamella catarrhalis},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3121668},
volume = 25,
year = 1987
}