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Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes

, , , , , , , and . Angewandte Chemie International Edition, 47 (33): 6172--6176 (August 2008)
DOI: 10.1002/anie.200802376

Abstract

(Graph Presented) Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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