The initial degradation rates (r) in human serum of three chitosans
with F-A = 0.42, 0.51, and 0.60 were determined by measuring the
decrease in viscosity as a function of time. A strong increase in
r with increasing F, of the chitosans was observed, with r increasing
proportionally to F-A(4.5). With increasing concentrations of lysozyme
added to the reaction mixtures of chitosan and serum, the relative
increase in degradation rate of chitosans with increasing F-A was
almost the same as that without lysozyme added. Addition of the chitinase
inhibitor allosamidin (50 mu M) did not inhibit the degradation rate
of chitosan (F-A = 0.60) by human serum. The results suggest that
chitosans are actually mainly depolymerized by lysozyme in human
serum, and not by other enzymes or other depolymerization mechanisms.
(C) 1997 Elsevier Science Ltd.
%0 Journal Article
%1 Varum1997a
%A Varum, K. M.
%A Myhr, M. M.
%A Hjerde, R. J. N.
%A Smidsrod, O.
%C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5
1GB
%D 1997
%I ELSEVIER SCI LTD
%J Carbohydr. Res.
%K ; ;; [ISI:] chitinase chitins chitosan; chitosans; deacetylated degradation; field lysozyme; nmr-spectroscopy serum;
%N 1-2
%P 99 -- 101
%T In vitro degradation rates of partially N-acetylated chitosans in
human serum
%V 299
%X The initial degradation rates (r) in human serum of three chitosans
with F-A = 0.42, 0.51, and 0.60 were determined by measuring the
decrease in viscosity as a function of time. A strong increase in
r with increasing F, of the chitosans was observed, with r increasing
proportionally to F-A(4.5). With increasing concentrations of lysozyme
added to the reaction mixtures of chitosan and serum, the relative
increase in degradation rate of chitosans with increasing F-A was
almost the same as that without lysozyme added. Addition of the chitinase
inhibitor allosamidin (50 mu M) did not inhibit the degradation rate
of chitosan (F-A = 0.60) by human serum. The results suggest that
chitosans are actually mainly depolymerized by lysozyme in human
serum, and not by other enzymes or other depolymerization mechanisms.
(C) 1997 Elsevier Science Ltd.
@article{Varum1997a,
__markedentry = {[phpts:6]},
abstract = {The initial degradation rates (r) in human serum of three chitosans
with F-A = 0.42, 0.51, and 0.60 were determined by measuring the
decrease in viscosity as a function of time. A strong increase in
r with increasing F, of the chitosans was observed, with r increasing
proportionally to F-A(4.5). With increasing concentrations of lysozyme
added to the reaction mixtures of chitosan and serum, the relative
increase in degradation rate of chitosans with increasing F-A was
almost the same as that without lysozyme added. Addition of the chitinase
inhibitor allosamidin (50 mu M) did not inhibit the degradation rate
of chitosan (F-A = 0.60) by human serum. The results suggest that
chitosans are actually mainly depolymerized by lysozyme in human
serum, and not by other enzymes or other depolymerization mechanisms.
(C) 1997 Elsevier Science Ltd.},
added-at = {2011-11-04T13:47:04.000+0100},
address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5
1GB},
author = {Varum, K. M. and Myhr, M. M. and Hjerde, R. J. N. and Smidsrod, O.},
biburl = {https://www.bibsonomy.org/bibtex/23a0655a59a54e97ef62e1e50986faf27/pawelsikorski},
citedref = {ANTHONSEN MW, 1993, CARBOHYD POLYM, V22, P193 ; DRAGET KI, 1992, BIOMATERIALS,
V13, P635 ; ESCOTT GM, 1995, INFECT IMMUN, V63, P4770 ; KOGA D, 1987,
AGR BIOL CHEM TOKYO, V51, P471 ; NORDTVEIT RJ, 1994, CARBOHYD POLYM,
V23, P253 ; NORDTVEIT RJ, 1996, CARBOHYD POLYM, V29, P163 ; OVERDIJK
B, 1994, GLYCOBIOLOGY, V4, P797 ; RENKEMA GH, 1995, J BIOL CHEM,
V270, P26252 ; SANNAN T, 1976, MAKROMOL CHEM, V177, P3589 ; STOKKE
BT, 1995, CAN J CHEM, V73, P1972 ; VARUM KM, 1991, CARBOHYD RES,
V211, P17 ; VARUM KM, 1991, CARBOHYD RES, V217, P19 ; VARUM KM, 1996,
BBA-GEN SUBJECTS, V1291, P5},
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intrahash = {3a0655a59a54e97ef62e1e50986faf27},
isifile-dt = {Article},
isifile-ga = {WT993},
isifile-j9 = {CARBOHYD RES},
isifile-nr = {13},
isifile-pi = {OXFORD},
isifile-rp = {Varum, KM, NORWEGIAN UNIV SCI & TECHNOL,DEPT BIOTECHNOL,NOBIPOL,N-7034
; TRONDHEIM,NORWAY.},
isifile-sc = {Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Organic},
isifile-tc = {24},
issn = {0008-6215},
journal = {Carbohydr. Res.},
keywords = {; ;; [ISI:] chitinase chitins chitosan; chitosans; deacetylated degradation; field lysozyme; nmr-spectroscopy serum;},
language = {English},
month = {MAR 26},
number = {1-2},
owner = {phpts},
pages = {99 -- 101},
publisher = {ELSEVIER SCI LTD},
size = {3 p.},
sourceid = {ISI:A1997WT99300013},
timestamp = {2011-11-04T13:47:27.000+0100},
title = {In vitro degradation rates of partially N-acetylated chitosans in
human serum},
volume = 299,
year = 1997
}