As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
%0 Journal Article
%1 Hsiao2014Design
%A Hsiao, Victoria
%A de los Santos, Emmanuel L. C.
%A Whitaker, Weston R.
%A Dueber, John E.
%A Murray, Richard M.
%D 2014
%I American Chemical Society
%J ACS Synth. Biol.
%K sensing synthetic-biology
%R 10.1021/sb500024b
%T Design and Implementation of a Biomolecular Concentration Tracker
%U http://dx.doi.org/10.1021/sb500024b
%X As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
@article{Hsiao2014Design,
abstract = {As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Hsiao, Victoria and de los Santos, Emmanuel L. C. and Whitaker, Weston R. and Dueber, John E. and Murray, Richard M.},
biburl = {https://www.bibsonomy.org/bibtex/2400d966a5eee9fde0f46f83eda6e25cb/karthikraman},
citeulike-article-id = {13172151},
citeulike-linkout-0 = {http://dx.doi.org/10.1021/sb500024b},
citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/sb500024b},
day = 6,
doi = {10.1021/sb500024b},
interhash = {33888d8e752a6cf6bac573e63a2fcd1c},
intrahash = {400d966a5eee9fde0f46f83eda6e25cb},
journal = {ACS Synth. Biol.},
keywords = {sensing synthetic-biology},
month = may,
posted-at = {2014-05-16 07:08:16},
priority = {2},
publisher = {American Chemical Society},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {Design and Implementation of a Biomolecular Concentration Tracker},
url = {http://dx.doi.org/10.1021/sb500024b},
year = 2014
}