Solvents effect on Spectrophotometric estimation of Bilastine
World Journal of Biology Pharmacy and Health Sciences, 15 (2):
195–201 (February 2024)DOI: 10.30574/wjbphs.2023.15.2.0344
Abstract
Bilastine is an anti-histamine used to treat Allergic reactions Two simple UV spectrophotometric methods have been developed for the estimation of Bilastine in bulk and pharmaceutical dosage forms. The absorption maxima (λmax) of Bilastine was found to be 280 nm in Methanol and in 0.1 N Acetic acid observed at 271 nm and Beer-Lambert’s law was obeyed over the concentration range 5-30µg/ml.LOD and LOQ values of Bilastine was found to be 0.102 µg/ml, 0.3097 µg/ml and 0.136 µg/ml, 0.411µg/ml in Methanol and 0.1N Acetic acid respectively. The validation parameters were treated statistically with ‘t’ test and significant differences were observed. The study clearly revealed that the solvents influence the determination of Bilastine. The methods developed were rapid and easy can be applied for the estimation of Bilastine in bulk and pharmaceutical formulations.