%0 Journal Article %1 WOS:000245021300018 %A de Mascena Diniz Maia, Maria %A de Vasconcelos, Elder Alpes %A de Mascena Diniz Maia, Paula Ferdinanda Conceicao %A da Costa Maciel, Jackeline %A Cajueiro, Karla Regina Rizzardi %A da Carvalho da Silva, Maria Paz %A da Silva Jr., Eronides Felisberto %A Dutra, Rosa Amalia Fireman %A Freire, Valder Nogueira %A de Lima Jr., Jose Luiz %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND %D 2007 %I ELSEVIER SCI LTD %J PROCESS BIOCHEMISTRY %K PEI; brasiliensis; glutaraldehyde} immobilization; polyethylenimine porous silicon; urease {Canavalia %N 3 %P 429-433 %R 10.1016/j.procbio.2006.08.008 %T Immobilization of urease on vapour phase stain etched porous silicon %V 42 %X Porous silicon layers fabricated by the reaction-induced vapor phase stain etch method were coated with 5% polyethylenimine. Urease from Canavalia brasiliensis beans was immobilized on this support through covalent linking with 2.5% glutaraldehyde. The pH and temperature profile of the immobilized and free urease exhibited higher activity at pH 6.5 and 37 degrees C. After being stored for 30 days at 4 degrees C, the immobilized enzyme had 75% of the initial activity. The maximum apparent Michaelis constant for free urease (K-m) was 94.33 mM whereas for immobilized urease was 53.04 mM. The maximum reaction velocity (V-max) for free urease was 3.51 mmol/min and for immobilized urease was 1.57 mmol/min. (c) 2006 Elsevier Ltd. All rights reserved.

@article{WOS:000245021300018, abstract = {Porous silicon layers fabricated by the reaction-induced vapor phase stain etch method were coated with 5% polyethylenimine. Urease from Canavalia brasiliensis beans was immobilized on this support through covalent linking with 2.5% glutaraldehyde. The pH and temperature profile of the immobilized and free urease exhibited higher activity at pH 6.5 and 37 degrees C. After being stored for 30 days at 4 degrees C, the immobilized enzyme had 75% of the initial activity. The maximum apparent Michaelis constant for free urease (K-m) was 94.33 mM whereas for immobilized urease was 53.04 mM. The maximum reaction velocity (V-max) for free urease was 3.51 mmol/min and for immobilized urease was 1.57 mmol/min. (c) 2006 Elsevier Ltd. All rights reserved.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND}, author = {de Mascena Diniz Maia, Maria and de Vasconcelos, Elder Alpes and de Mascena Diniz Maia, Paula Ferdinanda Conceicao and da Costa Maciel, Jackeline and Cajueiro, Karla Regina Rizzardi and da Carvalho da Silva, Maria Paz and da Silva Jr., Eronides Felisberto and Dutra, Rosa Amalia Fireman and Freire, Valder Nogueira and de Lima Jr., Jose Luiz}, biburl = {https://www.bibsonomy.org/bibtex/2568cad28d6d8bc360bd742b7fadd3f7e/ppgfis_ufc_br}, doi = {10.1016/j.procbio.2006.08.008}, interhash = {248f10862de5d1701936e26bdd806aa8}, intrahash = {568cad28d6d8bc360bd742b7fadd3f7e}, issn = {1359-5113}, journal = {PROCESS BIOCHEMISTRY}, keywords = {PEI; brasiliensis; glutaraldehyde} immobilization; polyethylenimine porous silicon; urease {Canavalia}, number = 3, pages = {429-433}, publisher = {ELSEVIER SCI LTD}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Immobilization of urease on vapour phase stain etched porous silicon}, tppubtype = {article}, volume = 42, year = 2007 }