Eighteen sequences complementary to less-conserved regions within the 16S and 23S ribosomal ribonucleic acid (rRNA) of Neisseria gonorrhoeae were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid (DNA): DNA hybridization format. Some of these probes exclusively detected Neisseria gonorrhoeae nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that rRNA-derived DNA-probes can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.
%0 Journal Article
%1 rossau_specific_1989
%A Rossau, R
%A Vanmechelen, E
%A Ley, J De
%A Heuverswijn, H Van
%D 1989
%J Journal of General Microbiology
%K Acid Bacterial, Conformation, Gonorrhea, Humans, Hybridization, Neisseria Neisseria, Nucleic Predictive Probes, Ribosomal, Species Specificity Tests, Value gonorrhoeae, of {DNA,} {DNA} {RNA,}
%N 6
%P 1735--1745
%T Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA
%U http://www.ncbi.nlm.nih.gov/pubmed/2482333
%V 135
%X Eighteen sequences complementary to less-conserved regions within the 16S and 23S ribosomal ribonucleic acid (rRNA) of Neisseria gonorrhoeae were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid (DNA): DNA hybridization format. Some of these probes exclusively detected Neisseria gonorrhoeae nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that rRNA-derived DNA-probes can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.
@article{rossau_specific_1989,
abstract = {Eighteen sequences complementary to less-conserved regions within the {16S} and {23S} ribosomal ribonucleic acid {(rRNA)} of Neisseria gonorrhoeae were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid {(DNA):} {DNA} hybridization format. Some of these probes exclusively detected Neisseria gonorrhoeae nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that {rRNA-derived} {DNA-probes} can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Rossau, R and Vanmechelen, E and Ley, J De and Heuverswijn, H Van},
biburl = {https://www.bibsonomy.org/bibtex/249cb98dddc4a140d3d0aa812e305717b/jelias},
interhash = {dbe34c585a22af73d70e9ce54fdb8c31},
intrahash = {49cb98dddc4a140d3d0aa812e305717b},
issn = {0022-1287},
journal = {Journal of General Microbiology},
keywords = {Acid Bacterial, Conformation, Gonorrhea, Humans, Hybridization, Neisseria Neisseria, Nucleic Predictive Probes, Ribosomal, Species Specificity Tests, Value gonorrhoeae, of {DNA,} {DNA} {RNA,}},
month = jun,
note = {{PMID:} 2482333},
number = 6,
pages = {1735--1745},
timestamp = {2011-03-11T10:06:44.000+0100},
title = {Specific Neisseria gonorrhoeae {DNA-probes} derived from ribosomal {RNA}},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2482333},
volume = 135,
year = 1989
}