%0 Journal Article %1 kmpfer_actinoplanes_2007 %A Kämpfer, Peter %A Huber, Birgit %A Thummes, Kathrin %A Grün-Wollny, Iris %A Busse, Hans-Jürgen %D 2007 %J International journal of systematic and evolutionary microbiology %K 16S Bacterial DNA IFZ Italy Micromonosporaceae Molecular_Sequence_Data Phylogeny RNA Ribosomal Soil_Microbiology %P 721-4 %T Actinoplanes couchii sp. nov %V 57 %X A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 \%), A. rectilineatus IFO 13941(T) (98.5 \%), A. palleronii JCM 7626(T) (97.8 \%), A. utahensis IFO 13244(T) (97.6 \%) and A. cyaneus DSM 46137(T) (97.6 \%). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 \%. Chemotaxonomic data major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH) supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)).

@article{kmpfer_actinoplanes_2007, abstract = {A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 \%), A. rectilineatus IFO 13941(T) (98.5 \%), A. palleronii JCM 7626(T) (97.8 \%), A. utahensis IFO 13244(T) (97.6 \%) and A. cyaneus DSM 46137(T) (97.6 \%). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 \%. Chemotaxonomic data [major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH)] supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)).}, added-at = {2008-05-07T16:02:06.000+0200}, author = {Kämpfer, Peter and Huber, Birgit and Thummes, Kathrin and Grün-Wollny, Iris and Busse, Hans-Jürgen}, biburl = {https://www.bibsonomy.org/bibtex/24a338f1f27faa6765dbbe515c56f8b6d/recymikrobio}, interhash = {b8d4840f130122750eee4ec2e17ae176}, intrahash = {4a338f1f27faa6765dbbe515c56f8b6d}, issn = {14665026}, journal = {International journal of systematic and evolutionary microbiology}, keywords = {16S Bacterial DNA IFZ Italy Micromonosporaceae Molecular_Sequence_Data Phylogeny RNA Ribosomal Soil_Microbiology}, month = {April}, note = {PMID: 17392194}, pages = {721-4}, timestamp = {2008-07-18T13:58:10.000+0200}, title = {Actinoplanes couchii sp. nov}, volume = 57, year = 2007 }