%0 Journal Article %1 Fischer2023.05.04.539359 %A Fischer, Sabine %A Schardt, Simon %A Lilao-Garzon, Joaquin %A Munoz-Descalzo, Silvia %D 2023 %I Cold Spring Harbor Laboratory %J bioRxiv %K cctb csi myown %P 2023.05.04.539359 %R 10.1101/2023.05.04.539359 %T The salt-and-pepper pattern in mouse blastocysts is compatible with signalling beyond the nearest neighbours. %U https://www.biorxiv.org/content/early/2023/05/05/2023.05.04.539359 %X Embryos develop in a concerted sequence of spatio-temporal arrangements of cells. In the preimplantation mouse embryo, the distribution of the cells in the inner cell mass evolves from a salt-and-pepper pattern to spatial segregation of two distinct cell types. The exact properties of the salt-and-pepper pattern have not been analysed so far. We investigate the spatio-temporal distribution of NANOG and GATA6 expressing cells in the ICM of the mouse blastocysts with quantitative three-dimensional single cell-based neighbourhood analyses. A combination of spatial statistics and agent-based modelling reveals that the cell fate distribution follows a local clustering pattern. Using ordinary differential equations modelling, we show that this pattern can be established by a distance-based signalling mechanism enabling cells to integrate information from the whole inner cell mass into their cell fate decision. Our work highlights the importance of longer-range signalling to ensure coordinated decisions in groups of cells to successfully build embryos.Competing Interest StatementThe authors have declared no competing interest.

@article{Fischer2023.05.04.539359, abstract = {Embryos develop in a concerted sequence of spatio-temporal arrangements of cells. In the preimplantation mouse embryo, the distribution of the cells in the inner cell mass evolves from a salt-and-pepper pattern to spatial segregation of two distinct cell types. The exact properties of the salt-and-pepper pattern have not been analysed so far. We investigate the spatio-temporal distribution of NANOG and GATA6 expressing cells in the ICM of the mouse blastocysts with quantitative three-dimensional single cell-based neighbourhood analyses. A combination of spatial statistics and agent-based modelling reveals that the cell fate distribution follows a local clustering pattern. Using ordinary differential equations modelling, we show that this pattern can be established by a distance-based signalling mechanism enabling cells to integrate information from the whole inner cell mass into their cell fate decision. Our work highlights the importance of longer-range signalling to ensure coordinated decisions in groups of cells to successfully build embryos.Competing Interest StatementThe authors have declared no competing interest.}, added-at = {2023-05-08T09:20:34.000+0200}, author = {Fischer, Sabine and Schardt, Simon and Lilao-Garzon, Joaquin and Munoz-Descalzo, Silvia}, biburl = {https://www.bibsonomy.org/bibtex/2516c6d4040300f756fb09337a45e7c7d/scfischer}, description = {The salt-and-pepper pattern in mouse blastocysts is compatible with signalling beyond the nearest neighbours. | bioRxiv}, doi = {10.1101/2023.05.04.539359}, elocation-id = {2023.05.04.539359}, eprint = {https://www.biorxiv.org/content/early/2023/05/05/2023.05.04.539359.full.pdf}, interhash = {83b7cfb2a02a1b79800c83b818cf44de}, intrahash = {516c6d4040300f756fb09337a45e7c7d}, journal = {bioRxiv}, keywords = {cctb csi myown}, pages = {2023.05.04.539359}, publisher = {Cold Spring Harbor Laboratory}, timestamp = {2023-05-08T09:20:34.000+0200}, title = {The salt-and-pepper pattern in mouse blastocysts is compatible with signalling beyond the nearest neighbours.}, url = {https://www.biorxiv.org/content/early/2023/05/05/2023.05.04.539359}, year = 2023 }