%0 Journal Article %1 Wang2009 %A Wang, Zhong %A Gerstein, Mark %A Snyder, Michael %D 2009 %J Nat Rev Genet %K highthroughput gene-expression %N 1 %P 57--63 %R 10.1038/nrg2484 %T RNA-Seq: a revolutionary tool for transcriptomics. %U http://dx.doi.org/10.1038/nrg2484 %V 10 %X RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

@article{Wang2009, abstract = {RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.}, added-at = {2013-03-07T22:09:43.000+0100}, author = {Wang, Zhong and Gerstein, Mark and Snyder, Michael}, biburl = {https://www.bibsonomy.org/bibtex/25213d3b989f305f672ab26311e7ef5ab/aorchid}, doi = {10.1038/nrg2484}, file = {:allorejection/NatRevGenet.10.57.pdf:PDF}, groups = {public}, institution = {Department of Molecular, Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, Connecticut 06520, USA.}, interhash = {d4e6fc1efc7df7048bea18359c00a593}, intrahash = {5213d3b989f305f672ab26311e7ef5ab}, journal = {Nat Rev Genet}, keywords = {highthroughput gene-expression}, language = {eng}, medline-pst = {ppublish}, month = Jan, number = 1, pages = {57--63}, pii = {nrg2484}, pmid = {19015660}, timestamp = {2013-03-07T22:09:43.000+0100}, title = {RNA-Seq: a revolutionary tool for transcriptomics.}, url = {http://dx.doi.org/10.1038/nrg2484}, username = {aorchid}, volume = 10, year = 2009 }