Morphological plasticity of Candida albicans is a major virulence factor. Using pH-dependent dimorphism we show, that human dendritic cells (DC) recognize filamentous forms and blastoconidia of a virulent C. albicans isolate (strain SC5314). Heat inactivated and viable blastoconidia are rapidly phagocytosed by human DC. However, viable yeast cells start to filament inside the DC at later stages of infection, leading to penetration and loss of cellular integrity. The cytokine burst of human DC induced upon contact with Candida is dominated by the granulocyte-activating, chemotactic factor IL-8 and the proinflammatory mediator TNF-alpha. Blastoconidia induce markedly lower cytokine levels than filamentous forms. Whereas IL-8 secretion is mainly cell mass dependent, release of TNF-alpha, a major proinflammatory cytokine, is clearly dependent on the morphology of Candida.
%0 Journal Article
%1 kurzai_polymorphism_2005
%A Kurzai, Oliver
%A Schmitt, Corinna
%A Bröcker, EvaB
%A Frosch, Matthias
%A Kolb-Mäurer, Annette
%D 2005
%J International Journal of Medical Microbiology: IJMM
%K Candida Cell Cells, Dendritic Differentiation, Factor-alpha, Factors Humans, Interleukin-12, Interleukin-8, Necrosis Phagocytosis, Tumor Virulence ag_kurzai albicans,
%N 2
%P 121--127
%T Polymorphism of Candida albicans is a major factor in the interaction with human dendritic cells
%U http://www.ncbi.nlm.nih.gov/pubmed/15969473
%V 295
%X Morphological plasticity of Candida albicans is a major virulence factor. Using pH-dependent dimorphism we show, that human dendritic cells (DC) recognize filamentous forms and blastoconidia of a virulent C. albicans isolate (strain SC5314). Heat inactivated and viable blastoconidia are rapidly phagocytosed by human DC. However, viable yeast cells start to filament inside the DC at later stages of infection, leading to penetration and loss of cellular integrity. The cytokine burst of human DC induced upon contact with Candida is dominated by the granulocyte-activating, chemotactic factor IL-8 and the proinflammatory mediator TNF-alpha. Blastoconidia induce markedly lower cytokine levels than filamentous forms. Whereas IL-8 secretion is mainly cell mass dependent, release of TNF-alpha, a major proinflammatory cytokine, is clearly dependent on the morphology of Candida.
@article{kurzai_polymorphism_2005,
abstract = {Morphological plasticity of Candida albicans is a major virulence factor. Using {pH-dependent} dimorphism we show, that human dendritic cells {(DC)} recognize filamentous forms and blastoconidia of a virulent C. albicans isolate (strain {SC5314).} Heat inactivated and viable blastoconidia are rapidly phagocytosed by human {DC.} However, viable yeast cells start to filament inside the {DC} at later stages of infection, leading to penetration and loss of cellular integrity. The cytokine burst of human {DC} induced upon contact with Candida is dominated by the granulocyte-activating, chemotactic factor {IL-8} and the proinflammatory mediator {TNF-alpha.} Blastoconidia induce markedly lower cytokine levels than filamentous forms. Whereas {IL-8} secretion is mainly cell mass dependent, release of {TNF-alpha,} a major proinflammatory cytokine, is clearly dependent on the morphology of Candida.},
added-at = {2011-04-07T15:44:20.000+0200},
author = {Kurzai, Oliver and Schmitt, Corinna and Bröcker, {EvaB} and Frosch, Matthias and {Kolb-Mäurer}, Annette},
biburl = {https://www.bibsonomy.org/bibtex/25f3d7e8aa41cae68c96025ade6f73212/hymi},
interhash = {161fadb5e3b44d8a75eb48ccc21f87bc},
intrahash = {5f3d7e8aa41cae68c96025ade6f73212},
issn = {1438-4221},
journal = {International Journal of Medical Microbiology: {IJMM}},
keywords = {Candida Cell Cells, Dendritic Differentiation, Factor-alpha, Factors Humans, Interleukin-12, Interleukin-8, Necrosis Phagocytosis, Tumor Virulence ag_kurzai albicans,},
month = jun,
note = {{PMID:} 15969473},
number = 2,
pages = {121--127},
timestamp = {2011-04-07T16:41:09.000+0200},
title = {Polymorphism of Candida albicans is a major factor in the interaction with human dendritic cells},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15969473},
volume = 295,
year = 2005
}