%0 Journal Article %1 kim_efficient_2019 %A Kim, Iana V. %A Ross, Eric J. %A Dietrich, Sascha %A Döring, Kristina %A Sánchez Alvarado, Alejandro %A Kuhn, Claus-D. %D 2019 %J BMC Genomics %K myown %N 1 %P 909 %R 10.1186/s12864-019-6292-y %T Efficient depletion of ribosomal RNA for RNA sequencing in planarians %U https://doi.org/10.1186/s12864-019-6292-y %V 20 %X The astounding regenerative abilities of planarian flatworms prompt steadily growing interest in examining their molecular foundation. Planarian regeneration was found to require hundreds of genes and is hence a complex process. Thus, RNA interference followed by transcriptome-wide gene expression analysis by RNA-seq is a popular technique to study the impact of any particular planarian gene on regeneration. Typically, the removal of ribosomal RNA (rRNA) is the first step of all RNA-seq library preparation protocols. To date, rRNA removal in planarians was primarily achieved by the enrichment of polyadenylated (poly(A)) transcripts. However, to better reflect transcriptome dynamics and to cover also non-poly(A) transcripts, a procedure for the targeted removal of rRNA in planarians is needed.

@article{kim_efficient_2019, abstract = {The astounding regenerative abilities of planarian flatworms prompt steadily growing interest in examining their molecular foundation. Planarian regeneration was found to require hundreds of genes and is hence a complex process. Thus, RNA interference followed by transcriptome-wide gene expression analysis by RNA-seq is a popular technique to study the impact of any particular planarian gene on regeneration. Typically, the removal of ribosomal RNA (rRNA) is the first step of all RNA-seq library preparation protocols. To date, rRNA removal in planarians was primarily achieved by the enrichment of polyadenylated (poly(A)) transcripts. However, to better reflect transcriptome dynamics and to cover also non-poly(A) transcripts, a procedure for the targeted removal of rRNA in planarians is needed.}, added-at = {2020-01-09T16:08:55.000+0100}, author = {Kim, Iana V. and Ross, Eric J. and Dietrich, Sascha and Döring, Kristina and Sánchez Alvarado, Alejandro and Kuhn, Claus-D.}, biburl = {https://www.bibsonomy.org/bibtex/26aa67eb5be61e1843810f07371b62dfc/cusysmed}, doi = {10.1186/s12864-019-6292-y}, file = {Kim et al. - 2019 - Efficient depletion of ribosomal RNA for RNA seque.pdf:/home/thorsten/Zotero/storage/FIDVNXRQ/Kim et al. - 2019 - Efficient depletion of ribosomal RNA for RNA seque.pdf:application/pdf}, interhash = {44a6042133ccc3826a126e1e2f5e5156}, intrahash = {6aa67eb5be61e1843810f07371b62dfc}, issn = {1471-2164}, journal = {BMC Genomics}, keywords = {myown}, month = nov, number = 1, pages = 909, timestamp = {2020-01-09T16:08:55.000+0100}, title = {Efficient depletion of ribosomal RNA for RNA sequencing in planarians}, url = {https://doi.org/10.1186/s12864-019-6292-y}, urldate = {2020-01-09}, volume = 20, year = 2019 }