Cold acclimation and BnCBF17-over-expression enhance photosynthetic performance and energy conversion efficiency during long-term growth of Brassica napus under elevated CO2 conditions
The effects of cold acclimation and long-term elevated CO 2 on photosynthetic performance of wild-type (WT) and BnCBF17 -over-expressing line of Brassica napus cv. Westar ( BnCBF17 -OE) grown at either 20/16 °C (non-acclimated) or 5/5 °C (cold acclimated) and at either ambient (380 μmol C mol −1 ) or elevated (700 μmol C mol −1 ) CO 2 were studied. Compared with non-acclimated WT, the BnCBF17 -OE grown at 20 °C mimicked the effects of cold acclimation on WT B. napus with respect to compact dwarf phenotype and increased rates of light-saturated CO 2 assimilation and photosynthetic electron transport. This was associated with enhanced energy conversion efficiency into biomass as assessed by decreased excitation pressure coupled to decreased dependence on non-photochemical energy dissipation for a given irradiance. Growth at elevated CO 2 decreased the light and CO 2 -saturated rates of photosynthesis by 30 \% for non-acclimated WT relative to growth at ambient CO 2 . This was associated with inhibition in electron transport rates (20 \%), decrease in amount of rbcL (35 \%) and cytosolic FBPase (70 \%) and increased excitation pressure and non-photochemical quenching in elevated versus ambient CO 2 -grown non-acclimated WT. In contrast, light and CO 2 -saturated rates of photosynthesis, electron transport, excitation pressure, non-photochemical quenching and levels of rbcL, cytosolic FBPase and Lhcb1 were insensitive to growth under elevated CO 2 in BnCBF17 -OE and cold-acclimated WT. Thus, BnCBF17 -over-expression and cold acclimation maintain enhanced energy conversion efficiency and reduced sensitivity to feedback-limited photosynthesis during long-term growth of B. napus under elevated CO 2 . Our results indicated that CBFs transcription factors regulate not only freezing tolerance but also has major whole plant effects.
%0 Journal Article
%1 citeulike:11005932
%A Dahal, Keshav
%A Gadapati, Winona
%A Savitch, LeonidV
%A Singh, Jas
%A Hüner, NormanP
%B Planta
%D 2012
%I Springer-Verlag
%J Planta
%K citeulikeExport co2, crop, ma16, protein
%N 5
%P 1639--1652
%R 10.1007/s00425-012-1710-2
%T Cold acclimation and BnCBF17-over-expression enhance photosynthetic performance and energy conversion efficiency during long-term growth of Brassica napus under elevated CO2 conditions
%U http://dx.doi.org/10.1007/s00425-012-1710-2
%V 236
%X The effects of cold acclimation and long-term elevated CO 2 on photosynthetic performance of wild-type (WT) and BnCBF17 -over-expressing line of Brassica napus cv. Westar ( BnCBF17 -OE) grown at either 20/16 °C (non-acclimated) or 5/5 °C (cold acclimated) and at either ambient (380 μmol C mol −1 ) or elevated (700 μmol C mol −1 ) CO 2 were studied. Compared with non-acclimated WT, the BnCBF17 -OE grown at 20 °C mimicked the effects of cold acclimation on WT B. napus with respect to compact dwarf phenotype and increased rates of light-saturated CO 2 assimilation and photosynthetic electron transport. This was associated with enhanced energy conversion efficiency into biomass as assessed by decreased excitation pressure coupled to decreased dependence on non-photochemical energy dissipation for a given irradiance. Growth at elevated CO 2 decreased the light and CO 2 -saturated rates of photosynthesis by 30 \% for non-acclimated WT relative to growth at ambient CO 2 . This was associated with inhibition in electron transport rates (20 \%), decrease in amount of rbcL (35 \%) and cytosolic FBPase (70 \%) and increased excitation pressure and non-photochemical quenching in elevated versus ambient CO 2 -grown non-acclimated WT. In contrast, light and CO 2 -saturated rates of photosynthesis, electron transport, excitation pressure, non-photochemical quenching and levels of rbcL, cytosolic FBPase and Lhcb1 were insensitive to growth under elevated CO 2 in BnCBF17 -OE and cold-acclimated WT. Thus, BnCBF17 -over-expression and cold acclimation maintain enhanced energy conversion efficiency and reduced sensitivity to feedback-limited photosynthesis during long-term growth of B. napus under elevated CO 2 . Our results indicated that CBFs transcription factors regulate not only freezing tolerance but also has major whole plant effects.
@article{citeulike:11005932,
abstract = {{The effects of cold acclimation and long-term elevated CO 2 on photosynthetic performance of wild-type (WT) and BnCBF17 -over-expressing line of Brassica napus cv. Westar ( BnCBF17 -OE) grown at either 20/16 °C (non-acclimated) or 5/5 °C (cold acclimated) and at either ambient (380 μmol C mol −1 ) or elevated (700 μmol C mol −1 ) CO 2 were studied. Compared with non-acclimated WT, the BnCBF17 -OE grown at 20 °C mimicked the effects of cold acclimation on WT B. napus with respect to compact dwarf phenotype and increased rates of light-saturated CO 2 assimilation and photosynthetic electron transport. This was associated with enhanced energy conversion efficiency into biomass as assessed by decreased excitation pressure coupled to decreased dependence on non-photochemical energy dissipation for a given irradiance. Growth at elevated CO 2 decreased the light and CO 2 -saturated rates of photosynthesis by 30 \% for non-acclimated WT relative to growth at ambient CO 2 . This was associated with inhibition in electron transport rates (20 \%), decrease in amount of rbcL (35 \%) and cytosolic FBPase (70 \%) and increased excitation pressure and non-photochemical quenching in elevated versus ambient CO 2 -grown non-acclimated WT. In contrast, light and CO 2 -saturated rates of photosynthesis, electron transport, excitation pressure, non-photochemical quenching and levels of rbcL, cytosolic FBPase and Lhcb1 were insensitive to growth under elevated CO 2 in BnCBF17 -OE and cold-acclimated WT. Thus, BnCBF17 -over-expression and cold acclimation maintain enhanced energy conversion efficiency and reduced sensitivity to feedback-limited photosynthesis during long-term growth of B. napus under elevated CO 2 . Our results indicated that CBFs transcription factors regulate not only freezing tolerance but also has major whole plant effects.}},
added-at = {2019-03-31T01:14:40.000+0100},
author = {Dahal, Keshav and Gadapati, Winona and Savitch, LeonidV and Singh, Jas and H\"{u}ner, NormanP},
biburl = {https://www.bibsonomy.org/bibtex/2748b3438923a0e8840c2e9adc4cc02c3/dianella},
booktitle = {Planta},
citeulike-article-id = {11005932},
citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00425-012-1710-2},
citeulike-linkout-1 = {http://www.springerlink.com/content/6133350213222k3t},
citeulike-linkout-2 = {http://link.springer.com/article/10.1007/s00425-012-1710-2},
day = 31,
doi = {10.1007/s00425-012-1710-2},
interhash = {87b8d33b516ad102eaf2c0115d664b1b},
intrahash = {748b3438923a0e8840c2e9adc4cc02c3},
issn = {0032-0935},
journal = {Planta},
keywords = {citeulikeExport co2, crop, ma16, protein},
month = jul,
number = 5,
pages = {1639--1652},
posted-at = {2016-01-14 02:30:04},
priority = {2},
publisher = {Springer-Verlag},
timestamp = {2019-03-31T01:16:26.000+0100},
title = {{Cold acclimation and BnCBF17-over-expression enhance photosynthetic performance and energy conversion efficiency during long-term growth of Brassica napus under elevated CO2 conditions}},
url = {http://dx.doi.org/10.1007/s00425-012-1710-2},
volume = 236,
year = 2012
}