10.1074/jbc.M808394200 The pineal gland plays an essential role in vertebrate chronobiology by converting time into a hormonal signal, melatonin, which is always elevated at night. Here we have analyzed the rodent pineal transcriptome using Affymetrix GeneChip® technology to obtain a more complete description of pineal cell biology. The effort revealed that 604 genes (1,268 probe sets) with Entrez Gene identifiers are differentially expressed greater than 2-fold between midnight and mid-day (false discovery rate <0.20). Expression is greater at night in ∼70\%. These findings were supported by the results of radiochemical in situ hybridization histology and quantitative real time-PCR studies. We also found that the regulatory mechanism controlling the night/day changes in the expression of most genes involves norepinephrine-cyclic AMP signaling. Comparison of the pineal gene expression profile with that in other tissues identified 334 genes (496 probe sets) that are expressed greater than 8-fold higher in the pineal gland relative to other tissues. Of these genes, 17\% are expressed at similar levels in the retina, consistent with a common evolutionary origin of these tissues. Functional categorization of the highly expressed and/or night/day differentially expressed genes identified clusters that are markers of specialized functions, including the immune/inflammation response, melatonin synthesis, photodetection, thyroid hormone signaling, and diverse aspects of cellular signaling and cell biology. These studies produce a paradigm shift in our understanding of the 24-h dynamics of the pineal gland from one focused on melatonin synthesis to one including many cellular processes. ER -
%0 Journal Article
%1 Bailey2009NightDay
%A Bailey, Michael J.
%A Coon, Steven L.
%A Carter, David A.
%A Humphries, Ann
%A Kim, Jong-so
%A Shi, Qiong
%A Gaildrat, Pascaline
%A Morin, Fabrice
%A Ganguly, Surajit
%A Hogenesch, John B.
%A Weller, Joan L.
%A Rath, Martin F.
%A Møller, Morten
%A Baler, Ruben
%A Sugden, David
%A Rangel, Zoila G.
%A Munson, Peter J.
%A Klein, David C.
%D 2009
%J Journal of Biological Chemistry
%K circadian-clock gene-expression
%N 12
%P 7606--7622
%R 10.1074/jbc.m808394200
%T Night/Day Changes in Pineal Expression of >600 Genes
%U http://dx.doi.org/10.1074/jbc.m808394200
%V 284
%X 10.1074/jbc.M808394200 The pineal gland plays an essential role in vertebrate chronobiology by converting time into a hormonal signal, melatonin, which is always elevated at night. Here we have analyzed the rodent pineal transcriptome using Affymetrix GeneChip® technology to obtain a more complete description of pineal cell biology. The effort revealed that 604 genes (1,268 probe sets) with Entrez Gene identifiers are differentially expressed greater than 2-fold between midnight and mid-day (false discovery rate <0.20). Expression is greater at night in ∼70\%. These findings were supported by the results of radiochemical in situ hybridization histology and quantitative real time-PCR studies. We also found that the regulatory mechanism controlling the night/day changes in the expression of most genes involves norepinephrine-cyclic AMP signaling. Comparison of the pineal gene expression profile with that in other tissues identified 334 genes (496 probe sets) that are expressed greater than 8-fold higher in the pineal gland relative to other tissues. Of these genes, 17\% are expressed at similar levels in the retina, consistent with a common evolutionary origin of these tissues. Functional categorization of the highly expressed and/or night/day differentially expressed genes identified clusters that are markers of specialized functions, including the immune/inflammation response, melatonin synthesis, photodetection, thyroid hormone signaling, and diverse aspects of cellular signaling and cell biology. These studies produce a paradigm shift in our understanding of the 24-h dynamics of the pineal gland from one focused on melatonin synthesis to one including many cellular processes. ER -
@article{Bailey2009NightDay,
abstract = {{10.1074/jbc.M808394200} The pineal gland plays an essential role in vertebrate chronobiology by converting time into a hormonal signal, melatonin, which is always elevated at night. Here we have analyzed the rodent pineal transcriptome using Affymetrix {GeneChip}® technology to obtain a more complete description of pineal cell biology. The effort revealed that 604 genes (1,268 probe sets) with Entrez Gene identifiers are differentially expressed greater than 2-fold between midnight and mid-day (false discovery rate <0.20). Expression is greater at night in ∼70\%. These findings were supported by the results of radiochemical in situ hybridization histology and quantitative real {time-PCR} studies. We also found that the regulatory mechanism controlling the night/day changes in the expression of most genes involves norepinephrine-cyclic {AMP} signaling. Comparison of the pineal gene expression profile with that in other tissues identified 334 genes (496 probe sets) that are expressed greater than 8-fold higher in the pineal gland relative to other tissues. Of these genes, 17\% are expressed at similar levels in the retina, consistent with a common evolutionary origin of these tissues. Functional categorization of the highly expressed and/or night/day differentially expressed genes identified clusters that are markers of specialized functions, including the immune/inflammation response, melatonin synthesis, photodetection, thyroid hormone signaling, and diverse aspects of cellular signaling and cell biology. These studies produce a paradigm shift in our understanding of the 24-h dynamics of the pineal gland from one focused on melatonin synthesis to one including many cellular processes. {ER} - },
added-at = {2018-12-02T16:09:07.000+0100},
author = {Bailey, Michael J. and Coon, Steven L. and Carter, David A. and Humphries, Ann and Kim, Jong-so and Shi, Qiong and Gaildrat, Pascaline and Morin, Fabrice and Ganguly, Surajit and Hogenesch, John B. and Weller, Joan L. and Rath, Martin F. and M{\o}ller, Morten and Baler, Ruben and Sugden, David and Rangel, Zoila G. and Munson, Peter J. and Klein, David C.},
biburl = {https://www.bibsonomy.org/bibtex/27646472929c97d14818a046ea7a39a48/karthikraman},
citeulike-article-id = {7512635},
citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.m808394200},
citeulike-linkout-1 = {http://www.jbc.org/content/284/12/7606.abstract},
citeulike-linkout-2 = {http://www.jbc.org/content/284/12/7606.full.pdf},
citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19103603},
citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19103603},
day = 20,
doi = {10.1074/jbc.m808394200},
interhash = {4f2d3b93420190295799a066af80f425},
intrahash = {7646472929c97d14818a046ea7a39a48},
journal = {Journal of Biological Chemistry},
keywords = {circadian-clock gene-expression},
month = mar,
number = 12,
pages = {7606--7622},
pmid = {19103603},
posted-at = {2010-07-19 16:46:34},
priority = {2},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {{Night/Day} Changes in Pineal Expression of >600 Genes},
url = {http://dx.doi.org/10.1074/jbc.m808394200},
volume = 284,
year = 2009
}