%0 Journal Article %1 bethge2014colorectal %A Bethge, Nicole %A Lothe, Ragnhild A %A Honne, Hilde %A Andresen, Kim %A Trøen, Gunhild %A Eknaes, Mette %A Liestøl, Knut %A Holte, Harald %A Delabie, Jan %A Smeland, Erlend B %A Lind, Guro E %D 2014 %J Epigenetics %K 2 background cancer epigenetics methylation %N 3 %P 428-436 %R 10.4161/epi.27554 %T Colorectal cancer DNA methylation marker panel validated with high performance in Non-Hodgkin lymphoma %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053461/ %V 9 %X Genes with altered DNA methylation can be used as biomarkers for cancer detection and assessment of prognosis. Here we analyzed the methylation status of a colorectal cancer biomarker panel (CNRIP1, FBN1, INA, MAL, SNCA, and SPG20) in 97 cancer cell lines, derived from 17 different cancer types. Interestingly, the genes were frequently methylated also in hematological cancer types and were therefore subjected to analyses in primary tumor samples from the major types of non-Hodgkin lymphomas (NHL) and in healthy controls. In total, the genes CNRIP1, FBN1, INA, MAL, SNCA, and SPG20 were methylated in 53%, 23%, 52%, 69%, 97%, and 92% of the tumor samples, respectively, and were unmethylated in all healthy controls. With the exception of a single tumor sample, a correct prediction of lymphoma or normal sample was made in a blinded analysis of the validation series using a combination of SNCA and SPG20. The combined ROC-curve analysis of these genes resulted in an area under the curve of 0.999 (P = 4.2 × 10−18), and a sensitivity and specificity of 98% and 100%, respectively, across the test and validation series. Interestingly, the promoter methylation of CNRIP1 was associated with decreased overall survival in diffuse large B-cell lymphoma (DLBCL) (P = 0.03). In conclusion, our results demonstrate that SNCA and SPG20 methylation might be suitable for early detection and monitoring of NHL. Furthermore, CNRIP1 could potentially be used as a prognostic factor in DLBCL.

@article{bethge2014colorectal, abstract = {Genes with altered DNA methylation can be used as biomarkers for cancer detection and assessment of prognosis. Here we analyzed the methylation status of a colorectal cancer biomarker panel (CNRIP1, FBN1, INA, MAL, SNCA, and SPG20) in 97 cancer cell lines, derived from 17 different cancer types. Interestingly, the genes were frequently methylated also in hematological cancer types and were therefore subjected to analyses in primary tumor samples from the major types of non-Hodgkin lymphomas (NHL) and in healthy controls. In total, the genes CNRIP1, FBN1, INA, MAL, SNCA, and SPG20 were methylated in 53%, 23%, 52%, 69%, 97%, and 92% of the tumor samples, respectively, and were unmethylated in all healthy controls. With the exception of a single tumor sample, a correct prediction of lymphoma or normal sample was made in a blinded analysis of the validation series using a combination of SNCA and SPG20. The combined ROC-curve analysis of these genes resulted in an area under the curve of 0.999 (P = 4.2 × 10−18), and a sensitivity and specificity of 98% and 100%, respectively, across the test and validation series. Interestingly, the promoter methylation of CNRIP1 was associated with decreased overall survival in diffuse large B-cell lymphoma (DLBCL) (P = 0.03). In conclusion, our results demonstrate that SNCA and SPG20 methylation might be suitable for early detection and monitoring of NHL. Furthermore, CNRIP1 could potentially be used as a prognostic factor in DLBCL.}, added-at = {2017-10-02T23:42:42.000+0200}, author = {Bethge, Nicole and Lothe, Ragnhild A and Honne, Hilde and Andresen, Kim and Trøen, Gunhild and Eknaes, Mette and Liestøl, Knut and Holte, Harald and Delabie, Jan and Smeland, Erlend B and Lind, Guro E}, biburl = {https://www.bibsonomy.org/bibtex/27c97bf923680b62f4d68f0eb49d70608/artheibault}, description = {This article describes the methylation profiles of several important cancer genes. I visited this article while looking for information on the SNPs identified by Kaushal et al. (2017).}, doi = {10.4161/epi.27554}, interhash = {31e7c9ba27d83da74c2a2bd80d3b463d}, intrahash = {7c97bf923680b62f4d68f0eb49d70608}, journal = {Epigenetics}, keywords = {2 background cancer epigenetics methylation}, month = mar, number = 3, pages = {428-436}, timestamp = {2017-10-26T15:07:27.000+0200}, title = {Colorectal cancer DNA methylation marker panel validated with high performance in Non-Hodgkin lymphoma}, url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053461/}, volume = 9, year = 2014 }