A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2 M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 mug mL-1 for imipenem and 1.5 and 20 mug mL-1 for rifampicin. The detection limits were 0.07 mug mL-1 and 0.47 mug mL-1 for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.
%0 Journal Article
%1 fernandez-torres_determination_2008
%A Fernández-Torres, R.
%A Bello-López, M.A.
%A Callejón-Mochón, M.
%A Jiménez-Sánchez, J.C.
%D 2008
%J Analytica Chimica Acta
%K High Imipenem, Mouse Rifampicin chromatography, liquid performance plasma,
%N 2
%P 204--210
%R 10.1016/j.aca.2007.12.026
%T Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection
%U http://www.sciencedirect.com/science/article/B6TF4-4RH37YJ-5/2/d704a0b3a6ebb5e6a9cf1cc65ebcd872
%V 608
%X A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2 M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 mug mL-1 for imipenem and 1.5 and 20 mug mL-1 for rifampicin. The detection limits were 0.07 mug mL-1 and 0.47 mug mL-1 for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.
@article{fernandez-torres_determination_2008,
abstract = {A high performance liquid chromatographic {(HPLC)} method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a {RP-18} column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2 M borate buffer {(pH} 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 [mu]g {mL-1} for imipenem and 1.5 and 20 [mu]g {mL-1} for rifampicin. The detection limits were 0.07 [mu]g {mL-1} and 0.47 [mu]g {mL-1} for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {{Fernández-Torres}, R. and {Bello-López}, {M.A.} and {Callejón-Mochón}, M. and {Jiménez-Sánchez}, {J.C.}},
biburl = {https://www.bibsonomy.org/bibtex/2830a8fe9ccdc247ee2919ccd9dc0281e/jelias},
doi = {10.1016/j.aca.2007.12.026},
interhash = {7cd21282472c4e29f5768b34b6d95efb},
intrahash = {830a8fe9ccdc247ee2919ccd9dc0281e},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
keywords = {High Imipenem, Mouse Rifampicin chromatography, liquid performance plasma,},
month = feb,
number = 2,
pages = {204--210},
timestamp = {2011-03-11T10:06:47.000+0100},
title = {Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection},
url = {http://www.sciencedirect.com/science/article/B6TF4-4RH37YJ-5/2/d704a0b3a6ebb5e6a9cf1cc65ebcd872},
volume = 608,
year = 2008
}