Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Here, we identify a fast signaling pathway for JA targeting the K+ efflux channel GORK. Wounding triggers both local and systemic stomatal closure by activation of the JA signaling cascade followed by GORK phosphorylation and activation through CBL1-CIPK5 Ca2+ sensor-kinase complexes. GORK activation strictly depends on plasma membrane targeting and Ca2+ binding of CBL1-CIPK5 complexes. Accordingly, in gork, cbl1, and cipk5 mutants, JA-induced stomatal closure is specifically abolished. The ABA-coreceptor ABI2 counteracts CBL1-CIPK5-dependent GORK activation. Hence, JA-induced Ca2+ signaling in response to biotic stress converges with the ABA-mediated drought stress pathway to facilitate GORK-mediated stomatal closure upon wounding.
Description
Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K+ Channels by a Ca2+ Sensor-Kinase CBL1-CIPK5 Complex. - PubMed - NCBI
%0 Journal Article
%1 Forster:2019:Dev-Cell:30528785
%A Förster, S
%A Schmidt, L K
%A Kopic, E
%A Anschütz, U
%A Huang, S
%A Schlücking, K
%A Köster, P
%A Waadt, R
%A Larrieu, A
%A Batistič, O
%A Rodriguez, P L
%A Grill, E
%A Kudla, J
%A Becker, D
%D 2019
%J Dev Cell
%K myown
%N 1
%P 87-99
%R 10.1016/j.devcel.2018.11.014
%T Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K+ Channels by a Ca2+ Sensor-Kinase CBL1-CIPK5 Complex
%U https://www.ncbi.nlm.nih.gov/pubmed/30528785
%V 48
%X Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Here, we identify a fast signaling pathway for JA targeting the K+ efflux channel GORK. Wounding triggers both local and systemic stomatal closure by activation of the JA signaling cascade followed by GORK phosphorylation and activation through CBL1-CIPK5 Ca2+ sensor-kinase complexes. GORK activation strictly depends on plasma membrane targeting and Ca2+ binding of CBL1-CIPK5 complexes. Accordingly, in gork, cbl1, and cipk5 mutants, JA-induced stomatal closure is specifically abolished. The ABA-coreceptor ABI2 counteracts CBL1-CIPK5-dependent GORK activation. Hence, JA-induced Ca2+ signaling in response to biotic stress converges with the ABA-mediated drought stress pathway to facilitate GORK-mediated stomatal closure upon wounding.
@article{Forster:2019:Dev-Cell:30528785,
abstract = {Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Here, we identify a fast signaling pathway for JA targeting the K+ efflux channel GORK. Wounding triggers both local and systemic stomatal closure by activation of the JA signaling cascade followed by GORK phosphorylation and activation through CBL1-CIPK5 Ca2+ sensor-kinase complexes. GORK activation strictly depends on plasma membrane targeting and Ca2+ binding of CBL1-CIPK5 complexes. Accordingly, in gork, cbl1, and cipk5 mutants, JA-induced stomatal closure is specifically abolished. The ABA-coreceptor ABI2 counteracts CBL1-CIPK5-dependent GORK activation. Hence, JA-induced Ca2+ signaling in response to biotic stress converges with the ABA-mediated drought stress pathway to facilitate GORK-mediated stomatal closure upon wounding.},
added-at = {2019-06-02T08:58:33.000+0200},
author = {F{\"o}rster, S and Schmidt, L K and Kopic, E and Ansch{\"u}tz, U and Huang, S and Schl{\"u}cking, K and K{\"o}ster, P and Waadt, R and Larrieu, A and Batistič, O and Rodriguez, P L and Grill, E and Kudla, J and Becker, D},
biburl = {https://www.bibsonomy.org/bibtex/283b086f62dcea55718f4640faa74acfd/dirkbecker},
description = {Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K+ Channels by a Ca2+ Sensor-Kinase CBL1-CIPK5 Complex. - PubMed - NCBI},
doi = {10.1016/j.devcel.2018.11.014},
interhash = {28520d3454e5e7ceaece1d4629e30cd4},
intrahash = {83b086f62dcea55718f4640faa74acfd},
journal = {Dev Cell},
keywords = {myown},
month = jan,
number = 1,
pages = {87-99},
pmid = {30528785},
timestamp = {2019-06-02T08:58:33.000+0200},
title = {Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K+ Channels by a Ca2+ Sensor-Kinase CBL1-CIPK5 Complex},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30528785},
volume = 48,
year = 2019
}