Expressed sequence tags (ESTs) have provided a first glimpse of the
collection of transcribed sequences in a variety of organisms. However,
a careful analysis of this sequence data can provide significant
additional functional, structural and evolutionary information. Our
analysis of the public EST sequences, available through the TIGR
Gene Indices (TGI; http://www.tigr.org/tdb/tdb.html ), is an attempt
to identify the genes represented by that data and to provide additional
information regarding those genes. Gene Indices are constructed for
selected organisms by first clustering, then assembling EST and annotated
gene sequences from GenBank. This process produces a set of unique,
high-fidelity virtual transcripts, or tentative consensus (TC) sequences.
The TC sequences can be used to provide putative genes with functional
annotation, to link the transcripts to mapping and genomic sequence
data, and to provide links between orthologous and paralogous genes.
%0 Journal Article
%1 Quackenbush:2000
%A Quackenbush, John
%A Liang, Feng
%A Holt, Ingeborg
%A Pertea, Geo
%A Upton, Jonathan
%D 2000
%J Nucleic Acids Research
%K imported
%N 1
%P 141--145
%T The TIGR Gene Indices: Reconstruction and representation of expressed
gene sequences
%U http://nar.oxfordjournals.org/cgi/content/abstract/28/1/141
%V 28
%X Expressed sequence tags (ESTs) have provided a first glimpse of the
collection of transcribed sequences in a variety of organisms. However,
a careful analysis of this sequence data can provide significant
additional functional, structural and evolutionary information. Our
analysis of the public EST sequences, available through the TIGR
Gene Indices (TGI; http://www.tigr.org/tdb/tdb.html ), is an attempt
to identify the genes represented by that data and to provide additional
information regarding those genes. Gene Indices are constructed for
selected organisms by first clustering, then assembling EST and annotated
gene sequences from GenBank. This process produces a set of unique,
high-fidelity virtual transcripts, or tentative consensus (TC) sequences.
The TC sequences can be used to provide putative genes with functional
annotation, to link the transcripts to mapping and genomic sequence
data, and to provide links between orthologous and paralogous genes.
@article{Quackenbush:2000,
abstract = {Expressed sequence tags (ESTs) have provided a first glimpse of the
collection of transcribed sequences in a variety of organisms. However,
a careful analysis of this sequence data can provide significant
additional functional, structural and evolutionary information. Our
analysis of the public EST sequences, available through the TIGR
Gene Indices (TGI; http://www.tigr.org/tdb/tdb.html ), is an attempt
to identify the genes represented by that data and to provide additional
information regarding those genes. Gene Indices are constructed for
selected organisms by first clustering, then assembling EST and annotated
gene sequences from GenBank. This process produces a set of unique,
high-fidelity virtual transcripts, or tentative consensus (TC) sequences.
The TC sequences can be used to provide putative genes with functional
annotation, to link the transcripts to mapping and genomic sequence
data, and to provide links between orthologous and paralogous genes.},
added-at = {2007-10-23T13:35:30.000+0200},
author = {Quackenbush, John and Liang, Feng and Holt, Ingeborg and Pertea, Geo and Upton, Jonathan},
biburl = {https://www.bibsonomy.org/bibtex/28e2b5bf573f1cc2c159d4e1f5974c611/tkirsten},
interhash = {9d5b049699b371b55fc61304b1c749a1},
intrahash = {8e2b5bf573f1cc2c159d4e1f5974c611},
journal = {Nucleic Acids Research},
keywords = {imported},
number = 1,
owner = {tkirsten},
pages = {141--145},
timestamp = {2007-10-23T13:35:40.000+0200},
title = {{The TIGR Gene Indices: Reconstruction and representation of expressed
gene sequences}},
url = {http://nar.oxfordjournals.org/cgi/content/abstract/28/1/141},
volume = 28,
year = 2000
}