%0 Journal Article %1 Tinnefeld2003 %A Tinnefeld, P %A Buschmann, V %A Weston, KD %A Sauer, M %C 1155 16TH ST, NW, WASHINGTON, DC 20036 USA %D 2003 %I AMER CHEMICAL SOC %J JOURNAL OF PHYSICAL CHEMISTRY A %K sauer %N 3 %P 323-327 %T Direct observation of collective blinking and energy transfer in a bichromophoric system %U http://dx.doi.org/10.1021/jp026565u %V 107 %X A bichromophoric model system-a short peptide labeled with tetramethylrhodamine (TMR) and the carbocyanine Cy5-embedded in poly(vinyl alcohol) (PVA) was used to investigate energy-transfer and collective blinking effects in multichromophoric systems at the level of single molecules. Experiments using direct excitation of the acceptor show evidence of photoinduced reverse intersystem crossing (T-1 --> T-N --> S-1 --> S-0) in single Cy5 molecules. We observed that even with the Cy5 fluorophore is in the triplet state, it continues to act as an energy transfer acceptor. This demonstrates that singlet-triplet energy transfer occurs, and can lead to efficient quenching of the fluorescence of the whole bichromophoric system.

@article{Tinnefeld2003, abstract = {A bichromophoric model system-a short peptide labeled with tetramethylrhodamine (TMR) and the carbocyanine Cy5-embedded in poly(vinyl alcohol) (PVA) was used to investigate energy-transfer and collective blinking effects in multichromophoric systems at the level of single molecules. Experiments using direct excitation of the acceptor show evidence of photoinduced reverse intersystem crossing (T-1 --> T-N --> S-1 --> S-0) in single Cy5 molecules. We observed that even with the Cy5 fluorophore is in the triplet state, it continues to act as an energy transfer acceptor. This demonstrates that singlet-triplet energy transfer occurs, and can lead to efficient quenching of the fluorescence of the whole bichromophoric system.}, added-at = {2011-03-01T10:57:13.000+0100}, address = {1155 16TH ST, NW, WASHINGTON, DC 20036 USA}, affiliation = {Sauer, M (Reprint Author), Univ Heidelberg, Inst Phys Chem, Neuenheimer Feld 253, D-69120 Heidelberg, Germany. Univ Heidelberg, Inst Phys Chem, D-69120 Heidelberg, Germany.}, author = {Tinnefeld, P and Buschmann, V and Weston, KD and Sauer, M}, biburl = {https://www.bibsonomy.org/bibtex/296db36bae48f38b4e9f48d93c361e2ff/reichert}, cited-references = {BOPP MA, 1997, P NATL ACAD SCI USA, V94, P10630. CHRIST T, 2001, ANGEW CHEM INT EDIT, V40, P4192. DEMPSTER DN, 1972, J CHEM SOC F2, V68, P1479. DIETRICH A, 2002, REV MOL BIOTECHNOL, V82, P211. ENGLISH DS, 2000, CHEM PHYS LETT, V324, P15. ENGLISH DS, 2000, J PHYS CHEM A, V104, P9057. FLEURY L, 2000, PHYS REV LETT, V84, P1148. FORSTER T, 1948, ANN PHYSIK, V2, P55. GENSCH T, 1999, ANGEW CHEM INT EDIT, V38, P3752. GRONHEID R, 2002, J AM CHEM SOC, V124, P2418. HA TJ, 1999, CHEM PHYS, V247, P107. HA TJ, 1999, P NATL ACAD SCI USA, V96, P893. HOFKENS J, 2000, J AM CHEM SOC, V122, P9278. HOFKENS J, 2001, SPECTROCHIM ACTA A, V57, P2093. HU DH, 1999, J AM CHEM SOC, V121, P6936. HUSER T, 2000, P NATL ACAD SCI USA, V97, P11187. JELEZKO F, 2000, J PHYS CHEM B, V104, P8093. LU HP, 1997, NATURE, V385, P143. MAUS M, 2001, J AM CHEM SOC, V123, P7668. MENZEL R, 1998, CHEM PHYS LETT, V291, P237. MENZEL R, 1999, PCCP PHYS CHEM CH PH, V1, P2435. SETH J, 1994, J AM CHEM SOC, V116, P10578. TIETZ C, 2001, BIOPHYS J, V81, P556. TINNEFELD P, 2000, SINGLE MOL, V1, P215. TINNEFELD P, 2001, J PHYS CHEM A, V105, P7989. VANDENBOUT DA, 1997, SCIENCE, V277, P1074. VEERMAN JA, 1999, PHYS REV LETT, V83, P2155. VOSCH T, 2001, ANGEW CHEM INT EDIT, V40, P4643. WESTON KD, 1999, CHEM PHYS LETT, V308, P58. WHITE JD, 2001, J CHEM PHYS, V114, P3848. WIDENGREN J, 2000, J PHYS CHEM A, V104, P6416. WIDENGREN J, 2000, PHYS CHEM CHEM PHYS, V2, P3435. YIP WT, 1998, J PHYS CHEM A, V102, P7564. YU J, 2000, SCIENCE, V289, P1327.}, doc-delivery-number = {638AD}, groups = {public}, interhash = {64d75d6e769ce8a3a206741dc98b8784}, intrahash = {96db36bae48f38b4e9f48d93c361e2ff}, issn = {1089-5639}, journal = {JOURNAL OF PHYSICAL CHEMISTRY A}, journal-iso = {J. Phys. Chem. A}, keywords = {sauer}, keywords-plus = {SINGLE-MOLECULE SPECTROSCOPY; FLUORESCENCE CORRELATION SPECTROSCOPY; PHOTOPHYSICAL DYNAMICS; CONJUGATED POLYMERS; ROOM-TEMPERATURE; DENDRIMERS; DYES; CHROMOPHORES; FLUCTUATIONS; SUBSTITUTION}, language = {English}, month = {JAN 23}, number = 3, number-of-cited-references = {34}, pages = {323-327}, publisher = {AMER CHEMICAL SOC}, subject-category = {Chemistry, Physical; Physics, Atomic, Molecular \& Chemical}, times-cited = {44}, timestamp = {2011-03-09T14:16:30.000+0100}, title = {Direct observation of collective blinking and energy transfer in a bichromophoric system}, type = {Letter}, unique-id = {ISI:000180545100001}, url = {http://dx.doi.org/10.1021/jp026565u}, username = {reichert}, volume = 107, year = 2003 }