%0 Journal Article %1 pitout_detection_2005 %A Pitout, Johann D D %A Gregson, Daniel B %A Poirel, Laurent %A McClure, Jo-Ann %A Le, Phillip %A Church, Deirdre L %D 2005 %J Journal of Clinical Microbiology %K Acid, Agents, Alberta, Bacterial, Chain Drug Edetic Health Humans, Imipenem, Laboratories, Microbial Phenotype, Planning, Polymerase Pseudomonas Reaction, Regional Resistance, Sensitivity Specificity, Tests, Thienamycins aeruginosa, and {Anti-Bacterial} {beta-Lactamases,} %N 7 %P 3129--3135 %R 43/7/3129 %T Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory %U http://www.ncbi.nlm.nih.gov/pubmed/16000424 %V 43 %X Metallo-beta-lactamases (MBLs) have been increasingly recognized from clinical isolates worldwide, but the laboratory detection of these strains is not well defined. We report a study that developed an EDTA disk screen test and a molecular diagnostic assay for the detection of MBL-producing Pseudomonas aeruginosa. Using NCCLS disk methodology, inhibition zone diameters were determined in tests with imipenem (IPM) and meropenem (MEM) disks alone and in combination with 930 microg of EDTA. This test was compared with the MBL Etest. The duplex PCR assay showed 100\% sensitivity and specificity for detecting MBL-producing control strains. Of the 241 clinical strains of IPM-nonsusceptible P. aeruginosa from the Calgary Health Region isolated from 2002 to 2004, 110/241 (46\%) were MBL positive using phenotypic methods while 107/241 (45\%) were PCR positive for MBL genes: 103/241 (43\%) for bla(VIM) and 4/241 (2\%) for bla(IMP). The EDTA disk screen test using MEM showed 100\% sensitivity and 97\% specificity for detecting MBLs in control and clinical strains. The EDTA disk screen test is simple to perform and to interpret and can easily be introduced into the workflow of a clinical laboratory. We recommend that all IPM-nonsusceptible P. aeruginosa isolates be routinely screened for MBL production using the EDTA disk screen test and that PCR confirmation be performed at a regional laboratory.

@article{pitout_detection_2005, abstract = {Metallo-beta-lactamases {(MBLs)} have been increasingly recognized from clinical isolates worldwide, but the laboratory detection of these strains is not well defined. We report a study that developed an {EDTA} disk screen test and a molecular diagnostic assay for the detection of {MBL-producing} Pseudomonas aeruginosa. Using {NCCLS} disk methodology, inhibition zone diameters were determined in tests with imipenem {(IPM)} and meropenem {(MEM)} disks alone and in combination with 930 microg of {EDTA.} This test was compared with the {MBL} Etest. The duplex {PCR} assay showed 100\% sensitivity and specificity for detecting {MBL-producing} control strains. Of the 241 clinical strains of {IPM-nonsusceptible} P. aeruginosa from the Calgary Health Region isolated from 2002 to 2004, 110/241 (46\%) were {MBL} positive using phenotypic methods while 107/241 (45\%) were {PCR} positive for {MBL} genes: 103/241 (43\%) for {bla(VIM)} and 4/241 (2\%) for {bla(IMP).} The {EDTA} disk screen test using {MEM} showed 100\% sensitivity and 97\% specificity for detecting {MBLs} in control and clinical strains. The {EDTA} disk screen test is simple to perform and to interpret and can easily be introduced into the workflow of a clinical laboratory. We recommend that all {IPM-nonsusceptible} P. aeruginosa isolates be routinely screened for {MBL} production using the {EDTA} disk screen test and that {PCR} confirmation be performed at a regional laboratory.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Pitout, Johann D D and Gregson, Daniel B and Poirel, Laurent and {McClure}, {Jo-Ann} and Le, Phillip and Church, Deirdre L}, biburl = {https://www.bibsonomy.org/bibtex/29eb64cd60721d963b11b3e46c31568e7/jelias}, doi = {43/7/3129}, interhash = {f8b0bd7fcb9884ee8414efbcae5ac05e}, intrahash = {9eb64cd60721d963b11b3e46c31568e7}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Acid, Agents, Alberta, Bacterial, Chain Drug Edetic Health Humans, Imipenem, Laboratories, Microbial Phenotype, Planning, Polymerase Pseudomonas Reaction, Regional Resistance, Sensitivity Specificity, Tests, Thienamycins aeruginosa, and {Anti-Bacterial} {beta-Lactamases,}}, month = jul, note = {{PMID:} 16000424}, number = 7, pages = {3129--3135}, timestamp = {2011-03-11T10:06:43.000+0100}, title = {Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory}, url = {http://www.ncbi.nlm.nih.gov/pubmed/16000424}, volume = 43, year = 2005 }