Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we show for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T cells have similar levels of immediate interferon (IFN) γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells have significantly higher cytotoxicity against fluA virus-infected cells compared with their CD56(-) counterparts, whereas both subsets have similar IFN-γ responses. We further demonstrate that the CD16-dependent degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity, contribute to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T-cell subsets during fluA virus infection and may help improve the γδ T-cell-based immunotherapy for viral infection.
%0 Journal Article
%1 Qin2012a
%A Qin, Gang
%A Liu, Yinping
%A Zheng, Jian
%A Xiang, Zheng
%A Ng, Iris H Y.
%A Malik Peiris, J. S.
%A Lau, Yu-Lung
%A Tu, Wenwei
%D 2012
%J J Infect Dis
%K facs mucosal pneumonia tlymphocytes virus
%N 11
%P 1646--1653
%R 10.1093/infdis/jis253
%T Phenotypic and Functional Characterization of Human γδ T-Cell Subsets in Response to Influenza A Viruses.
%U http://dx.doi.org/10.1093/infdis/jis253
%V 205
%X Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we show for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T cells have similar levels of immediate interferon (IFN) γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells have significantly higher cytotoxicity against fluA virus-infected cells compared with their CD56(-) counterparts, whereas both subsets have similar IFN-γ responses. We further demonstrate that the CD16-dependent degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity, contribute to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T-cell subsets during fluA virus infection and may help improve the γδ T-cell-based immunotherapy for viral infection.
@article{Qin2012a,
abstract = {Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we show for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T cells have similar levels of immediate interferon (IFN) γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells have significantly higher cytotoxicity against fluA virus-infected cells compared with their CD56(-) counterparts, whereas both subsets have similar IFN-γ responses. We further demonstrate that the CD16-dependent degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity, contribute to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T-cell subsets during fluA virus infection and may help improve the γδ T-cell-based immunotherapy for viral infection.},
added-at = {2012-05-18T02:20:16.000+0200},
author = {Qin, Gang and Liu, Yinping and Zheng, Jian and Xiang, Zheng and Ng, Iris H Y. and {Malik Peiris}, J. S. and Lau, Yu-Lung and Tu, Wenwei},
biburl = {https://www.bibsonomy.org/bibtex/2a27ad866c6b2fc49f71eef90723dd8c4/aorchid},
doi = {10.1093/infdis/jis253},
file = {:allorejection/nkt/JInfectDis.205.1646.pdf:PDF},
groups = {public},
institution = {Departments of Paediatrics and Adolescent Medicine.},
interhash = {1289f976d2d69e3ea9646ef68cc02574},
intrahash = {a27ad866c6b2fc49f71eef90723dd8c4},
journal = {J Infect Dis},
keywords = {facs mucosal pneumonia tlymphocytes virus},
language = {eng},
medline-pst = {ppublish},
month = Jun,
number = 11,
pages = {1646--1653},
pii = {jis253},
pmid = {22457284},
timestamp = {2012-05-18T02:20:16.000+0200},
title = {Phenotypic and Functional Characterization of Human γδ T-Cell Subsets in Response to Influenza A Viruses.},
url = {http://dx.doi.org/10.1093/infdis/jis253},
username = {aorchid},
volume = 205,
year = 2012
}