The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, sigmaB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine sigmaB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify sigmaB-dependent genes at different growth phases.We detected 105 sigmaB-positively regulated genes and 111 genes which appeared to be under negative control of sigmaB and validated 36 sigmaB-positively regulated genes in vivo using a reporter gene fusion system.Genes comprising the sigmaB regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. Ten of the sigmaB-positively regulated genes of L. monocytogenes are absent in L. innocua. A total of 75 sigmaB-positively regulated listerial genes had homologs in B. subtilis, but only 33 have been previously described as being sigmaB-regulated in B. subtilis even though both species share a highly conserved sigmaB-dependent consensus sequence. A low overlap of genes may reflects adaptation of these bacteria to their respective environmental conditions.
Institute for Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, D-35392 Giessen, Germany. torsten.hain@mikrobio.med.uni-giessen.de
%0 Journal Article
%1 Hain2008
%A Hain, Torsten
%A Hossain, Hamid
%A Chatterjee, Som S.
%A Machata, Silke
%A Volk, Ute
%A Wagner, Sandra
%A Brors, Benedikt
%A Haas, Stefan
%A Kuenne, Carsten T.
%A Billion, Andre
%A Otten, Sonja
%A Pane-Farre, Jan
%A Engelmann, Susanne
%A Chakraborty, Trinad
%D 2008
%J BMC Microbiol
%K /&/ Acids Analysis, Array Bacterial, Bile Binding Biological Chromosomes, Concentration; Developmental, Energy Expression Factor, Gene Genetic, Genomics, Immunoblotting; Listeria Metabolism, Oligonucleotide Osmolar Profiling, Regulation, Regulon, Salts, Sequence Sigma Sites; Transcription, Transport, and development/metabolism; drug effects effects; genetics/growth genetics/metabolism/physiology; genetics; methods; monocytogenes, pharmacology;
%P 20
%R 10.1186/1471-2180-8-20
%T Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon.
%U http://dx.doi.org/10.1186/1471-2180-8-20
%V 8
%X The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, sigmaB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine sigmaB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify sigmaB-dependent genes at different growth phases.We detected 105 sigmaB-positively regulated genes and 111 genes which appeared to be under negative control of sigmaB and validated 36 sigmaB-positively regulated genes in vivo using a reporter gene fusion system.Genes comprising the sigmaB regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. Ten of the sigmaB-positively regulated genes of L. monocytogenes are absent in L. innocua. A total of 75 sigmaB-positively regulated listerial genes had homologs in B. subtilis, but only 33 have been previously described as being sigmaB-regulated in B. subtilis even though both species share a highly conserved sigmaB-dependent consensus sequence. A low overlap of genes may reflects adaptation of these bacteria to their respective environmental conditions.
@article{Hain2008,
__markedentry = {[bbrors:6]},
abstract = {The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, sigmaB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine sigmaB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify sigmaB-dependent genes at different growth phases.We detected 105 sigmaB-positively regulated genes and 111 genes which appeared to be under negative control of sigmaB and validated 36 sigmaB-positively regulated genes in vivo using a reporter gene fusion system.Genes comprising the sigmaB regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. Ten of the sigmaB-positively regulated genes of L. monocytogenes are absent in L. innocua. A total of 75 sigmaB-positively regulated listerial genes had homologs in B. subtilis, but only 33 have been previously described as being sigmaB-regulated in B. subtilis even though both species share a highly conserved sigmaB-dependent consensus sequence. A low overlap of genes may reflects adaptation of these bacteria to their respective environmental conditions.},
added-at = {2015-04-09T12:36:21.000+0200},
author = {Hain, Torsten and Hossain, Hamid and Chatterjee, Som S. and Machata, Silke and Volk, Ute and Wagner, Sandra and Brors, Benedikt and Haas, Stefan and Kuenne, Carsten T. and Billion, Andre and Otten, Sonja and Pane-Farre, Jan and Engelmann, Susanne and Chakraborty, Trinad},
biburl = {https://www.bibsonomy.org/bibtex/2a7a21373a3a9b682d831132446f819f2/bbrors},
doi = {10.1186/1471-2180-8-20},
institution = {Institute for Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, D-35392 Giessen, Germany. torsten.hain@mikrobio.med.uni-giessen.de},
interhash = {566e2e22d7dbbfb0d1f826e319e4f6d2},
intrahash = {a7a21373a3a9b682d831132446f819f2},
journal = {BMC Microbiol},
keywords = {/&/ Acids Analysis, Array Bacterial, Bile Binding Biological Chromosomes, Concentration; Developmental, Energy Expression Factor, Gene Genetic, Genomics, Immunoblotting; Listeria Metabolism, Oligonucleotide Osmolar Profiling, Regulation, Regulon, Salts, Sequence Sigma Sites; Transcription, Transport, and development/metabolism; drug effects effects; genetics/growth genetics/metabolism/physiology; genetics; methods; monocytogenes, pharmacology;},
language = {eng},
medline-pst = {epublish},
owner = {bbrors},
pages = 20,
pii = {1471-2180-8-20},
pmid = {18226246},
timestamp = {2015-04-09T12:36:21.000+0200},
title = {Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon.},
url = {http://dx.doi.org/10.1186/1471-2180-8-20},
volume = 8,
year = 2008
}