Mature neocortical layers all derive from the cortical plate (CP), a
transient zone in the dorsal telencephalon into which young neurons are
continuously delivered. To understand cytogenetic and histogenetic events that
trigger the emergence of the CP, we have used a slice culture technique. Most
divisions at the ventricular surface generated paired cycling daughters (P/P
divisions) and the majority of the P/P divisions were asymmetric in daughter
cell behavior; they frequently sent one daughter cell to a non-surface (NS)
position, the subventricular zone (SVZ), within a single cell-cycle length
while keeping the other mitotic daughter for division at the surface. The
NS-dividing cells were mostly Hu+ and their daughters were also
Hu+, suggesting their commitment to the neuronal lineage and supply
of early neurons at a position much closer to their destiny than from the
ventricular surface. The release of a cycling daughter cell to SVZ was
achieved by collapse of the ventricular process of the cell, followed by its
NS division. Neurogenin2 (Ngn2) was immunohistochemically detected in a
certain cycling population during G1 phase and was further restricted during
G2-M phases to the SVZ-directed population. Its retroviral introduction
converted surface divisions to NS divisions. The asymmetric P/P division may
therefore contribute to efficient neuron/progenitor segregation required for
CP initiation through cell cycle-dependent and lineage-restricted expression
of Ngn2.
Description
Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells
%0 Journal Article
%1 miyata2004asymmetric
%A Miyata, Takaki
%A Kawaguchi, Ayano
%A Saito, Kanako
%A Kawano, Masako
%A Muto, Tetsuji
%A Ogawa, Masaharu
%D 2004
%J Development
%K migration neurons npc phd
%N 13
%P 3133--3145
%R 10.1242/dev.01173
%T Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells
%U http://dev.biologists.org/content/131/13/3133.abstract
%V 131
%X Mature neocortical layers all derive from the cortical plate (CP), a
transient zone in the dorsal telencephalon into which young neurons are
continuously delivered. To understand cytogenetic and histogenetic events that
trigger the emergence of the CP, we have used a slice culture technique. Most
divisions at the ventricular surface generated paired cycling daughters (P/P
divisions) and the majority of the P/P divisions were asymmetric in daughter
cell behavior; they frequently sent one daughter cell to a non-surface (NS)
position, the subventricular zone (SVZ), within a single cell-cycle length
while keeping the other mitotic daughter for division at the surface. The
NS-dividing cells were mostly Hu+ and their daughters were also
Hu+, suggesting their commitment to the neuronal lineage and supply
of early neurons at a position much closer to their destiny than from the
ventricular surface. The release of a cycling daughter cell to SVZ was
achieved by collapse of the ventricular process of the cell, followed by its
NS division. Neurogenin2 (Ngn2) was immunohistochemically detected in a
certain cycling population during G1 phase and was further restricted during
G2-M phases to the SVZ-directed population. Its retroviral introduction
converted surface divisions to NS divisions. The asymmetric P/P division may
therefore contribute to efficient neuron/progenitor segregation required for
CP initiation through cell cycle-dependent and lineage-restricted expression
of Ngn2.
@article{miyata2004asymmetric,
abstract = {Mature neocortical layers all derive from the cortical plate (CP), a
transient zone in the dorsal telencephalon into which young neurons are
continuously delivered. To understand cytogenetic and histogenetic events that
trigger the emergence of the CP, we have used a slice culture technique. Most
divisions at the ventricular surface generated paired cycling daughters (P/P
divisions) and the majority of the P/P divisions were asymmetric in daughter
cell behavior; they frequently sent one daughter cell to a non-surface (NS)
position, the subventricular zone (SVZ), within a single cell-cycle length
while keeping the other mitotic daughter for division at the surface. The
NS-dividing cells were mostly Hu+ and their daughters were also
Hu+, suggesting their commitment to the neuronal lineage and supply
of early neurons at a position much closer to their destiny than from the
ventricular surface. The release of a cycling daughter cell to SVZ was
achieved by collapse of the ventricular process of the cell, followed by its
NS division. Neurogenin2 (Ngn2) was immunohistochemically detected in a
certain cycling population during G1 phase and was further restricted during
G2-M phases to the SVZ-directed population. Its retroviral introduction
converted surface divisions to NS divisions. The asymmetric P/P division may
therefore contribute to efficient neuron/progenitor segregation required for
CP initiation through cell cycle-dependent and lineage-restricted expression
of Ngn2.},
added-at = {2014-10-08T11:45:11.000+0200},
author = {Miyata, Takaki and Kawaguchi, Ayano and Saito, Kanako and Kawano, Masako and Muto, Tetsuji and Ogawa, Masaharu},
biburl = {https://www.bibsonomy.org/bibtex/2a84e6315cc845e4b36eede75fde62747/bkoch},
description = {Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells},
doi = {10.1242/dev.01173},
eprint = {http://dev.biologists.org/content/131/13/3133.full.pdf+html},
interhash = {bbd40ff80493c58be4888dcf99add4a8},
intrahash = {a84e6315cc845e4b36eede75fde62747},
journal = {Development},
keywords = {migration neurons npc phd},
number = 13,
pages = {3133--3145},
timestamp = {2014-10-08T11:45:11.000+0200},
title = {Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells},
url = {http://dev.biologists.org/content/131/13/3133.abstract},
volume = 131,
year = 2004
}