%0 Journal Article %1 Wen2017Enhanced %A Wen, Zhiqiang %A Minton, Nigel P. %A Zhang, Ying %A Li, Qi %A Liu, Jinle %A Jiang, Yu %A Yang, Sheng %D 2017 %J Metabolic engineering %K consortia metabolic-engineering %P 38--48 %T Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. %U http://view.ncbi.nlm.nih.gov/pubmed/27794465 %V 39 %X The efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a lignocellulosic feedstock using a single process microorganism has yet to be demonstrated. Herein, we developed a consolidated bioprocessing (CBP) based on a twin-clostridial consortium composed of Clostridium cellulovorans and Clostridium beijerinckii capable of producing cellulosic butanol from alkali-extracted, deshelled corn cobs (AECC). To accomplish this a genetic system was developed for C. cellulovorans and used to knock out the genes encoding acetate kinase (Clocel\_1892) and lactate dehydrogenase (Clocel\_1533), and to overexpress the gene encoding butyrate kinase (Clocel\_3674), thereby pulling carbon flux towards butyrate production. In parallel, to enhance ethanol production, the expression of a putative hydrogenase gene (Clocel\_2243) was down-regulated using CRISPR interference (CRISPRi). Simultaneously, genes involved in organic acids reassimilation (ctfAB, cbei\_3833/3834) and pentose utilization (xylR, cbei\_2385 and xylT, cbei\_0109) were engineered in C. beijerinckii to enhance solvent production. The engineered twin-clostridia consortium was shown to decompose 83.2g/L of AECC and produce 22.1g/L of solvents (4.25g/L acetone, 11.5g/L butanol and 6.37g/L ethanol). This titer of acetone-butanol-ethanol (ABE) approximates to that achieved from a starchy feedstock. The developed twin-clostridial consortium serves as a promising platform for ABE fermentation from lignocellulose by CBP. Copyright \copyright 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

@article{Wen2017Enhanced, abstract = {The efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a lignocellulosic feedstock using a single process microorganism has yet to be demonstrated. Herein, we developed a consolidated bioprocessing ({CBP}) based on a twin-clostridial consortium composed of Clostridium cellulovorans and Clostridium beijerinckii capable of producing cellulosic butanol from alkali-extracted, deshelled corn cobs ({AECC}). To accomplish this a genetic system was developed for C. cellulovorans and used to knock out the genes encoding acetate kinase (Clocel\_1892) and lactate dehydrogenase (Clocel\_1533), and to overexpress the gene encoding butyrate kinase (Clocel\_3674), thereby pulling carbon flux towards butyrate production. In parallel, to enhance ethanol production, the expression of a putative hydrogenase gene (Clocel\_2243) was down-regulated using {CRISPR} interference ({CRISPRi}). Simultaneously, genes involved in organic acids reassimilation ({ctfAB}, cbei\_3833/3834) and pentose utilization ({xylR}, cbei\_2385 and {xylT}, cbei\_0109) were engineered in C. beijerinckii to enhance solvent production. The engineered twin-clostridia consortium was shown to decompose {83.2g/L} of {AECC} and produce {22.1g/L} of solvents ({4.25g/L} acetone, {11.5g/L} butanol and {6.37g/L} ethanol). This titer of acetone-butanol-ethanol ({ABE}) approximates to that achieved from a starchy feedstock. The developed twin-clostridial consortium serves as a promising platform for {ABE} fermentation from lignocellulose by {CBP}. Copyright {\copyright} 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Wen, Zhiqiang and Minton, Nigel P. and Zhang, Ying and Li, Qi and Liu, Jinle and Jiang, Yu and Yang, Sheng}, biburl = {https://www.bibsonomy.org/bibtex/2b7c67179315c8f2180244620eafaecf8/karthikraman}, citeulike-article-id = {14380930}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/27794465}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=27794465}, interhash = {eab16c2de940f3709b2e53c8584ecb6b}, intrahash = {b7c67179315c8f2180244620eafaecf8}, issn = {1096-7184}, journal = {Metabolic engineering}, keywords = {consortia metabolic-engineering}, month = jan, pages = {38--48}, pmid = {27794465}, posted-at = {2017-06-22 12:47:19}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Enhanced solvent production by metabolic engineering of a twin-clostridial consortium.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/27794465}, volume = 39, year = 2017 }