%0 Journal Article %1 RN1029 %A Dindas, J. %A Scherzer, S. %A Roelfsema, M. R. G. %A von Meyer, K. %A Müller, H. M. %A Al-Rasheid, K. A. S. %A Palme, K. %A Dietrich, P. %A Becker, D. %A Bennett, M. J. %A Hedrich, R. %D 2018 %J Nature Communications %K arabidopsis myOwn root %R ARTN 1174 10.1038/s41467-018-03582-5 %T AUX1-mediated root hair auxin influx governs SCF -type Ca signaling %U /brokenurl#<Go to ISI>://WOS:000427929200005 %V 9 %X Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration-and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA(-) triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots.

@article{RN1029, abstract = {Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration-and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA(-) triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Dindas, J. and Scherzer, S. and Roelfsema, M. R. G. and von Meyer, K. and Müller, H. M. and Al-Rasheid, K. A. S. and Palme, K. and Dietrich, P. and Becker, D. and Bennett, M. J. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/2ba02167de391158a79c8bb64401966b8/rainerhedrich_2}, doi = {ARTN 1174 10.1038/s41467-018-03582-5}, interhash = {35c24a595b3b13fff4c8b575c2a6c161}, intrahash = {ba02167de391158a79c8bb64401966b8}, issn = {2041-1723}, journal = {Nature Communications}, keywords = {arabidopsis myOwn root}, note = {Fz9kk Times Cited:137 Cited References Count:52}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {AUX1-mediated root hair auxin influx governs SCF -type Ca signaling}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000427929200005}, volume = 9, year = 2018 }