A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was \textgreater99\% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.
%0 Journal Article
%1 lam_new_2011
%A Lâm, Thiên-Trí
%A Elias, Johannes
%A Frosch, Matthias
%A Vogel, Ulrich
%A Claus, Heike
%D 2011
%J International Journal of Medical Microbiology
%K Bacterial Genotype, Haemophilus Serotype capsule, influenzae, primer, {DNA}
%R 10.1016/j.ijmm.2010.07.004
%T New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142
%U http://www.sciencedirect.com/science/article/B7GW0-516R015-4/2/9c1baef3ac030745feb676261d25e20a
%V In Press, Corrected Proof
%X A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was \textgreater99\% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.
@article{lam_new_2011,
abstract = {A new {PCR} protocol for molecular typing of Haemophilus influenzae serotype e {(Hie)} was developed. To this end, the sequence of the cap region {II} of Hie strain {ATCC8142} was identified, which was {\textgreater}99\% identical to the recently published sequence of Hie isolate 274. The {PCR} using primer pair {TTL63/TTL64} amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all {non-Hie} strains, there were no false positives. False-negative results of the {PCR} proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Lâm, {Thiên-Trí} and Elias, Johannes and Frosch, Matthias and Vogel, Ulrich and Claus, Heike},
biburl = {https://www.bibsonomy.org/bibtex/2ba53ed97c1d1e4f69132263334243d8f/jelias},
doi = {10.1016/j.ijmm.2010.07.004},
interhash = {e098689a78df8c4a5cecbc92a6606f06},
intrahash = {ba53ed97c1d1e4f69132263334243d8f},
issn = {1438-4221},
journal = {International Journal of Medical Microbiology},
keywords = {Bacterial Genotype, Haemophilus Serotype capsule, influenzae, primer, {DNA}},
timestamp = {2011-03-11T10:05:50.000+0100},
title = {New diagnostic {PCR} for Haemophilus influenzae serotype e based on the cap locus of strain {ATCC} 8142},
url = {http://www.sciencedirect.com/science/article/B7GW0-516R015-4/2/9c1baef3ac030745feb676261d25e20a},
volume = {In Press, Corrected Proof},
year = 2011
}