Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL −1 of blood in 80% of patients with metastatic prostate cancer ( n = 20), in 70% of patients with metastatic breast cancer ( n = 30), in 50% of patients with metastatic pancreatic cancer ( n = 18), and in 0% of normal controls ( n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.
Description
Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers - Abstract - Physical Biology - IOPscience
%0 Journal Article
%1 marrinucci2012fluid
%A Marrinucci, Dena
%A Bethel, Kelly
%A Kolatkar, Anand
%A Luttgen, Madelyn S
%A Malchiodi, Michael
%A Baehring, Franziska
%A Voigt, Katharina
%A Lazar, Daniel
%A Nieva, Jorge
%A Bazhenova, Lyudmila
%A Ko, Andrew H
%A Korn, W Michael
%A Schram, Ethan
%A Coward, Michael
%A Yang, Xing
%A Metzner, Thomas
%A Lamy, Rachelle
%A Honnatti, Meghana
%A Yoshioka, Craig
%A Kunken, Joshua
%A Petrova, Yelena
%A Sok, Devin
%A Nelson, David
%A Kuhn, Peter
%D 2012
%J Physical Biology
%K CTCs biopsy cells circulating fluid metastatic tumor
%N 1
%P 016003
%T Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers
%U http://stacks.iop.org/1478-3975/9/i=1/a=016003
%V 9
%X Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL −1 of blood in 80% of patients with metastatic prostate cancer ( n = 20), in 70% of patients with metastatic breast cancer ( n = 30), in 50% of patients with metastatic pancreatic cancer ( n = 18), and in 0% of normal controls ( n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.
@article{marrinucci2012fluid,
abstract = {Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL −1 of blood in 80% of patients with metastatic prostate cancer ( n = 20), in 70% of patients with metastatic breast cancer ( n = 30), in 50% of patients with metastatic pancreatic cancer ( n = 18), and in 0% of normal controls ( n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.},
added-at = {2012-04-16T04:27:21.000+0200},
author = {Marrinucci, Dena and Bethel, Kelly and Kolatkar, Anand and Luttgen, Madelyn S and Malchiodi, Michael and Baehring, Franziska and Voigt, Katharina and Lazar, Daniel and Nieva, Jorge and Bazhenova, Lyudmila and Ko, Andrew H and Korn, W Michael and Schram, Ethan and Coward, Michael and Yang, Xing and Metzner, Thomas and Lamy, Rachelle and Honnatti, Meghana and Yoshioka, Craig and Kunken, Joshua and Petrova, Yelena and Sok, Devin and Nelson, David and Kuhn, Peter},
biburl = {https://www.bibsonomy.org/bibtex/2c096bfb0f0d0723a827015480c85a992/rodrimauricio},
description = {Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers - Abstract - Physical Biology - IOPscience},
interhash = {000ee42ae07a5e0f01dc583035651939},
intrahash = {c096bfb0f0d0723a827015480c85a992},
journal = {Physical Biology},
keywords = {CTCs biopsy cells circulating fluid metastatic tumor},
number = 1,
pages = 016003,
timestamp = {2012-04-16T04:27:22.000+0200},
title = {Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers},
url = {http://stacks.iop.org/1478-3975/9/i=1/a=016003},
volume = 9,
year = 2012
}