Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phenanthrol ine-2,9-dicarboxylic acid (BCPDA). The loss of lytic activity was the highest for Th-K7-Mel. Th-G1-Mel retains almost the same lytic activity as Mel. A homogeneous liposome time-resolved fluoroimmunoassay (LITRFIA) of Th in serum has been carried out with Th-G1-Mel between 5 ng and 10 microg.
%0 Journal Article
%1 citeulike:613250
%A Ius, A.
%A Bacigalupo, M. A.
%A Longhi, R.
%A Meroni, G.
%C Istituto di Biocatalisi e Riconoscimento Molecolare, C.N.R., Milan, Italy. iusWico.mi.cnr.it
%D 2000
%J Fresenius J Anal Chem
%K lytic_peptide immunoassay liposome mellitin
%N 8
%P 869--872
%T Selectively conjugated melittins for liposome time-resolved fluoroimmunoassay of theophylline in serum.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11227424
%V 366
%X Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phenanthrol ine-2,9-dicarboxylic acid (BCPDA). The loss of lytic activity was the highest for Th-K7-Mel. Th-G1-Mel retains almost the same lytic activity as Mel. A homogeneous liposome time-resolved fluoroimmunoassay (LITRFIA) of Th in serum has been carried out with Th-G1-Mel between 5 ng and 10 microg.
@article{citeulike:613250,
abstract = {Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phenanthrol ine-2,9-dicarboxylic acid (BCPDA). The loss of lytic activity was the highest for Th-K7-Mel. Th-G1-Mel retains almost the same lytic activity as Mel. A homogeneous liposome time-resolved fluoroimmunoassay (LITRFIA) of Th in serum has been carried out with Th-G1-Mel between 5 ng and 10 microg.},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Istituto di Biocatalisi e Riconoscimento Molecolare, C.N.R., Milan, Italy. iusWico.mi.cnr.it},
author = {Ius, A. and Bacigalupo, M. A. and Longhi, R. and Meroni, G.},
biburl = {https://www.bibsonomy.org/bibtex/2da6a8fedfd5832158792638971ec8469/biblio24},
citeulike-article-id = {613250},
interhash = {bccf958d7fa048e8632771d801ef0a37},
intrahash = {da6a8fedfd5832158792638971ec8469},
issn = {0937-0633},
journal = {Fresenius J Anal Chem},
keywords = {lytic_peptide immunoassay liposome mellitin},
month = {April},
number = 8,
pages = {869--872},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Selectively conjugated melittins for liposome time-resolved fluoroimmunoassay of theophylline in serum.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=11227424},
volume = 366,
year = 2000
}