%0 Journal Article %1 Gao2010116 %A Gao, Ting %A Yao, Hui %A Song, Jingyuan %A Liu, Chang %A Zhu, Yingjie %A Ma, Xinye %A Pang, Xiaohui %A Xu, Hongxi %A Chen, Shilin %D 2010 %J Journal of Ethnopharmacology %K ITS2 fabaceae identification medicinal phd %N 1 %P 116 - 121 %R 10.1016/j.jep.2010.04.026 %T Identification of medicinal plants in the family Fabaceae using a potential \DNA\ barcode \ITS2\ %U http://www.sciencedirect.com/science/article/pii/S0378874110002576 %V 130 %X Aim of the study To test whether the \ITS2\ region is an effective marker for use in authenticating of the family Fabaceae which contains many important medicinal plants. Materials and methods The \ITS2\ regions of 114 samples in Fabaceae were amplified. Sequence assembly was assembled by CodonCode Aligner V3.0. In combination with sequences from public database, the sequences were aligned by Clustal W, and genetic distances were computed using \MEGA\ V4.0. The intra- vs. inter-specific variations were assessed by six metrics, wilcoxon two-sample tests and “barcoding gaps”. Species identification was accomplished using TaxonGAP V2.4, \BLAST1\ and the nearest distance method. Results \ITS2\ sequences had considerable variation at the genus and species level. The intra-specific divergence ranged from 0% to 14.4%, with an average of 1.7%, and the inter-specific divergence ranged from 0% to 63.0%, with an average of 8.6%. Twenty-four species found in the Chinese Pharmacopoeia, along with another 66 species including their adulterants, were successfully identified based on \ITS2\ sequences. In addition, \ITS2\ worked well, with over 80.0% of species and 100% of genera being correctly differentiated for the 1507 sequences derived from 1126 species belonging to 196 genera. Conclusions Our findings support the notion that \ITS2\ can be used as an efficient and powerful marker and a potential barcode to distinguish various species in Fabaceae.

@article{Gao2010116, abstract = {Aim of the study To test whether the \{ITS2\} region is an effective marker for use in authenticating of the family Fabaceae which contains many important medicinal plants. Materials and methods The \{ITS2\} regions of 114 samples in Fabaceae were amplified. Sequence assembly was assembled by CodonCode Aligner V3.0. In combination with sequences from public database, the sequences were aligned by Clustal W, and genetic distances were computed using \{MEGA\} V4.0. The intra- vs. inter-specific variations were assessed by six metrics, wilcoxon two-sample tests and “barcoding gaps”. Species identification was accomplished using TaxonGAP V2.4, \{BLAST1\} and the nearest distance method. Results \{ITS2\} sequences had considerable variation at the genus and species level. The intra-specific divergence ranged from 0% to 14.4%, with an average of 1.7%, and the inter-specific divergence ranged from 0% to 63.0%, with an average of 8.6%. Twenty-four species found in the Chinese Pharmacopoeia, along with another 66 species including their adulterants, were successfully identified based on \{ITS2\} sequences. In addition, \{ITS2\} worked well, with over 80.0% of species and 100% of genera being correctly differentiated for the 1507 sequences derived from 1126 species belonging to 196 genera. Conclusions Our findings support the notion that \{ITS2\} can be used as an efficient and powerful marker and a potential barcode to distinguish various species in Fabaceae. }, added-at = {2013-12-16T18:39:47.000+0100}, author = {Gao, Ting and Yao, Hui and Song, Jingyuan and Liu, Chang and Zhu, Yingjie and Ma, Xinye and Pang, Xiaohui and Xu, Hongxi and Chen, Shilin}, biburl = {https://www.bibsonomy.org/bibtex/26b9a81c3fc3f0f809597c2c8d4ef5688/drgirasol}, description = {Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2}, doi = {10.1016/j.jep.2010.04.026}, interhash = {2da8af3e11f1810c83b9a75bfb37fb9a}, intrahash = {6b9a81c3fc3f0f809597c2c8d4ef5688}, issn = {0378-8741}, journal = {Journal of Ethnopharmacology }, keywords = {ITS2 fabaceae identification medicinal phd}, number = 1, pages = {116 - 121}, timestamp = {2013-12-16T18:39:47.000+0100}, title = {Identification of medicinal plants in the family Fabaceae using a potential \{DNA\} barcode \{ITS2\} }, url = {http://www.sciencedirect.com/science/article/pii/S0378874110002576}, volume = 130, year = 2010 }