Simultaneous detection of Haemophilus influenzae type b polysaccharide-specific antibodies and Neisseria meningitidis serogroup A, C, Y, and W-135 polysaccharide-specific antibodies in a fluorescent-bead-based multiplex immunoassay
We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R=0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.
%0 Journal Article
%1 de_voer_simultaneous_2009
%A de Voer, Richarda M
%A Schepp, Rutger M
%A Versteegh, Florens G A
%A van der Klis, Fiona R M
%A Berbers, Guy A M
%D 2009
%J Clinical and Vaccine Immunology: CVI
%K A, Antibodies, Antigens, Bacterial, C, Cross Fluorescence, G, Haemophilus Humans, Immunoassay, Immunoglobulin Microspheres, Neisseria Polysaccharides, Reactions, Sensitivity Serogroup Specificity W-135, Y, and b, influenzae meningitidis, type
%N 3
%P 433--436
%R 10.1128/CVI.00364-08
%T Simultaneous detection of Haemophilus influenzae type b polysaccharide-specific antibodies and Neisseria meningitidis serogroup A, C, Y, and W-135 polysaccharide-specific antibodies in a fluorescent-bead-based multiplex immunoassay
%U http://www.ncbi.nlm.nih.gov/pubmed/19129470
%V 16
%X We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R=0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.
@article{de_voer_simultaneous_2009,
abstract = {We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay {(MIA)} to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide {(HibPS).} The monoplex {HibPS} assay was compared to a {HibPS-specific} competitive enzyme-linked immunosorbent assay and showed a good correlation {(R=0.96).} Furthermore, no cross-reactivity between {HibPS} and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib {MIA} is a useful tool to investigate serological responses toward different childhood {PS} vaccines.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {de Voer, Richarda M and Schepp, Rutger M and Versteegh, Florens G A and van der Klis, Fiona R M and Berbers, Guy A M},
biburl = {https://www.bibsonomy.org/bibtex/2e98361d0685ede23b8ff0e44d5804f22/jelias},
doi = {10.1128/CVI.00364-08},
interhash = {35b88dbd67353fc3958c2b47306d9d86},
intrahash = {e98361d0685ede23b8ff0e44d5804f22},
issn = {{1556-679X}},
journal = {Clinical and Vaccine Immunology: {CVI}},
keywords = {A, Antibodies, Antigens, Bacterial, C, Cross Fluorescence, G, Haemophilus Humans, Immunoassay, Immunoglobulin Microspheres, Neisseria Polysaccharides, Reactions, Sensitivity Serogroup Specificity W-135, Y, and b, influenzae meningitidis, type},
month = mar,
note = {{PMID:} 19129470},
number = 3,
pages = {433--436},
timestamp = {2011-03-11T10:06:41.000+0100},
title = {Simultaneous detection of Haemophilus influenzae type b polysaccharide-specific antibodies and Neisseria meningitidis serogroup A, C, Y, and W-135 polysaccharide-specific antibodies in a fluorescent-bead-based multiplex immunoassay},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19129470},
volume = 16,
year = 2009
}