A complex regulatory network controls aerobic ethanol oxidation in Pseudomonas aeruginosa:indication of four levels of sensor kinases and response regulators
Microbiology, (January 2010)DOI: 10.1099/mic.0.032847-0
Abstract
Abstract revised:In addition to the known response regulator ErbR (former AgmR) and the two component regulatory system EraSR (former ExaDE), three additional regulatory proteins have been identified to be involved in controlling transcription of the aerobic ethanol oxidation system in Pseudomonas aeruginosa. Two putative sensor kinases, ErcS and ErcS', and a response regulator ErdR were found, all of which show significant similarity to the two component flhSR system controlling methanol and formaldehyde metabolism in Paracoccus denitrificans. All three identified response regulators, EraR (former ExaE), ErbR (former AgmR) and ErdR, are members of the luxR family. The three sensor kinases EraS (former ExaD), ErcS and ErcS' do not contain a membrane domain. Apparently they are localised in the cytoplasm and recognise cytoplasmic signals. Inactivation of gene ercS caused an extended lag phase on ethanol. Inactivation of both genes, ercS and ercS', resulted in no growth at all on ethanol, as did inactivation of gene erdR.Of the three sensor kinases and three response regulators identified thus far, only the EraSR (former ExaDE) system forms a corresponding kinase/regulator pair. Using reporter gene constructs of all identified regulatory genes in different mutants allowed to establish the hierarchy of a hypothetical complex regulatory network. Probably two additional sensor kinases and two additional response regulators, which are hidden among the numerous regulatory genes annotated in the genome of P. aeruginosa, remain to be identified.
Description
A complex regulatory network controls aerobic etha... [Microbiology. 2010] - PubMed result