A sensitive spectrophotometric method for the determination of hydroxylamine
is described. The method is based on the oxidation of hydroxylamine
to nitrite using sodium arsenate under alkaline condition. The formed
nitrite is determined based on the diazo coupling reaction between
p-nitroaniline and N-(1-naphthyl)ethylenediamine dihydrochloride
NEDA. The system obeys Beer's law over the concentration range
0-7 microg of hydroxylamine at 545 nm and the colour is stable for
3 h. The molar absorptivity of the colour system is found to be
6.7 x 10(4) l mol(-1) cm(-1). The relative standard deviation is
1.2% for ten determinations at 4 microg of hydroxylamine. Interferences
due to various foreign ions have been studied and the method has
been applied to the determination of hydroxylamine and its derivatives
used in pharmaceutical formulations after hydrolysis.
%0 Journal Article
%1 citeulike:499927
%A Deepa, B.
%A Balasubramanian, N.
%A Nagaraja, K. S.
%C Department of Chemistry, Loyola Institute of Frontier Energy, Loyola
College, Chennai, India.
%D 2004
%J Chem Pharm Bull (Tokyo)
%K hydroxylamine spectroscopy analysis
%N 12
%P 1473--1475
%T Spectrophotometric determination of hydroxylamine and its derivatives
in pharmaceuticals.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15577248
%V 52
%X A sensitive spectrophotometric method for the determination of hydroxylamine
is described. The method is based on the oxidation of hydroxylamine
to nitrite using sodium arsenate under alkaline condition. The formed
nitrite is determined based on the diazo coupling reaction between
p-nitroaniline and N-(1-naphthyl)ethylenediamine dihydrochloride
NEDA. The system obeys Beer's law over the concentration range
0-7 microg of hydroxylamine at 545 nm and the colour is stable for
3 h. The molar absorptivity of the colour system is found to be
6.7 x 10(4) l mol(-1) cm(-1). The relative standard deviation is
1.2% for ten determinations at 4 microg of hydroxylamine. Interferences
due to various foreign ions have been studied and the method has
been applied to the determination of hydroxylamine and its derivatives
used in pharmaceutical formulations after hydrolysis.
@article{citeulike:499927,
abstract = {A sensitive spectrophotometric method for the determination of hydroxylamine
is described. The method is based on the oxidation of hydroxylamine
to nitrite using sodium arsenate under alkaline condition. The formed
nitrite is determined based on the diazo coupling reaction between
p-nitroaniline and N-(1-naphthyl)ethylenediamine dihydrochloride
[NEDA]. The system obeys Beer's law over the concentration range
0-7 microg of hydroxylamine at 545 nm and the colour is stable for
3 h. The molar absorptivity of the colour system is found to be
6.7 x 10(4) l mol(-1) cm(-1). The relative standard deviation is
1.2% for ten determinations at 4 microg of hydroxylamine. Interferences
due to various foreign ions have been studied and the method has
been applied to the determination of hydroxylamine and its derivatives
used in pharmaceutical formulations after hydrolysis.},
added-at = {2007-02-02T11:54:15.000+0100},
address = {Department of Chemistry, Loyola Institute of Frontier Energy, Loyola
College, Chennai, India.},
author = {Deepa, B. and Balasubramanian, N. and Nagaraja, K. S.},
biburl = {https://www.bibsonomy.org/bibtex/2f3ac7d2a092c44cda141e94acc93aa76/robert},
citeulike-article-id = {499927},
interhash = {def8c23315009cbb315364e6a48d7c28},
intrahash = {f3ac7d2a092c44cda141e94acc93aa76},
issn = {0009-2363},
journal = {Chem Pharm Bull (Tokyo)},
keywords = {hydroxylamine spectroscopy analysis},
month = {December},
number = 12,
pages = {1473--1475},
priority = {2},
timestamp = {2007-02-02T11:54:15.000+0100},
title = {Spectrophotometric determination of hydroxylamine and its derivatives
in pharmaceuticals.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=15577248},
volume = 52,
year = 2004
}