Modelling the growth limits (growth/no growth interface) of Escherichia
coli as a function of temperature, pH, lactic acid concentration,
and water activity.
The form of a previously developed B��lehr��dek type of growth rate
model was used to develop a probability model for defining the growth/no
growth interface as a function of temperature (10 to 37 degrees
C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM),
and water activity (0.955 to 0.999; NaCl was used as the humectant).
Escherichia coli was unable to grow in broth in which the undissociated
lactic acid concentration exceeded 11 mM or, with two exceptions,
at a pH of 3.9 or less with no lactic acid present. Under experimental
conditions at which the pH and the undissociated acid concentrations
were the major growth-limiting factors, the growth/no growth interface
was essentially independent of temperature at temperatures ranging
from 15 to 37 degrees C. The interface between conditions that allowed
growth and conditions at which growth did not occur was abrupt.
The inhibitory effect of combinations of water activity and pH varied
with temperature. Predictions of the model for the growth/no growth
interface were consistent with 95% of the experimental data set.
%0 Journal Article
%1 citeulike:498792
%A Presser, K. A.
%A Ross, T.
%A Ratkowsky, D. A.
%C Department of Agricultural Science, University of Tasmania, Hobart,
Australia. kirsty.presser@utas.edu.au
%D 1998
%J Appl Environ Microbiol
%K growth ecoli
%N 5
%P 1773--1779
%T Modelling the growth limits (growth/no growth interface) of Escherichia
coli as a function of temperature, pH, lactic acid concentration,
and water activity.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9572950
%V 64
%X The form of a previously developed B��lehr��dek type of growth rate
model was used to develop a probability model for defining the growth/no
growth interface as a function of temperature (10 to 37 degrees
C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM),
and water activity (0.955 to 0.999; NaCl was used as the humectant).
Escherichia coli was unable to grow in broth in which the undissociated
lactic acid concentration exceeded 11 mM or, with two exceptions,
at a pH of 3.9 or less with no lactic acid present. Under experimental
conditions at which the pH and the undissociated acid concentrations
were the major growth-limiting factors, the growth/no growth interface
was essentially independent of temperature at temperatures ranging
from 15 to 37 degrees C. The interface between conditions that allowed
growth and conditions at which growth did not occur was abrupt.
The inhibitory effect of combinations of water activity and pH varied
with temperature. Predictions of the model for the growth/no growth
interface were consistent with 95% of the experimental data set.
@article{citeulike:498792,
abstract = {The form of a previously developed B��lehr��dek type of growth rate
model was used to develop a probability model for defining the growth/no
growth interface as a function of temperature (10 to 37 degrees
C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM),
and water activity (0.955 to 0.999; NaCl was used as the humectant).
Escherichia coli was unable to grow in broth in which the undissociated
lactic acid concentration exceeded 11 mM or, with two exceptions,
at a pH of 3.9 or less with no lactic acid present. Under experimental
conditions at which the pH and the undissociated acid concentrations
were the major growth-limiting factors, the growth/no growth interface
was essentially independent of temperature at temperatures ranging
from 15 to 37 degrees C. The interface between conditions that allowed
growth and conditions at which growth did not occur was abrupt.
The inhibitory effect of combinations of water activity and pH varied
with temperature. Predictions of the model for the growth/no growth
interface were consistent with 95% of the experimental data set.},
added-at = {2007-02-02T11:54:15.000+0100},
address = {Department of Agricultural Science, University of Tasmania, Hobart,
Australia. kirsty.presser@utas.edu.au},
author = {Presser, K. A. and Ross, T. and Ratkowsky, D. A.},
biburl = {https://www.bibsonomy.org/bibtex/2f45e2f9cfc6f54903b47468e0a1afb2c/robert},
citeulike-article-id = {498792},
interhash = {dc0682a044728d84b0325114752b4106},
intrahash = {f45e2f9cfc6f54903b47468e0a1afb2c},
issn = {0099-2240},
journal = {Appl Environ Microbiol},
keywords = {growth ecoli},
month = May,
number = 5,
pages = {1773--1779},
priority = {2},
timestamp = {2007-02-02T11:54:15.000+0100},
title = {Modelling the growth limits (growth/no growth interface) of Escherichia
coli as a function of temperature, pH, lactic acid concentration,
and water activity.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9572950},
volume = 64,
year = 1998
}