%0 Journal Article %1 citeulike:4381133 %A Stoddart, David %A Heron, Andrew J J. %A Mikhailova, Ellina %A Maglia, Giovanni %A Bayley, Hagan %D 2009 %J Proceedings of the National Academy of Sciences of the United States of America %K imported %R 10.1073/pnas.0901054106 %T Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore. %U http://dx.doi.org/10.1073/pnas.0901054106 %X The sequencing of individual DNA strands with nanopores is under investigation as a rapid, low-cost platform in which bases are identified in order as the DNA strand is transported through a pore under an electrical potential. Although the preparation of solid-state nanopores is improving, biological nanopores, such as alpha-hemolysin (alphaHL), are advantageous because they can be precisely manipulated by genetic modification. Here, we show that the transmembrane beta- barrel of an engineered alphaHL pore contains 3 recognition sites that can be used to identify all 4 DNA bases in an immobilized single-stranded DNA molecule, whether they are located in an otherwise homopolymeric DNA strand or in a heteropolymeric strand. The additional steps required to enable nanopore DNA sequencing are outlined.

@article{citeulike:4381133, abstract = {The sequencing of individual DNA strands with nanopores is under investigation as a rapid, low-cost platform in which bases are identified in order as the DNA strand is transported through a pore under an electrical potential. Although the preparation of solid-state nanopores is improving, biological nanopores, such as alpha-hemolysin (alphaHL), are advantageous because they can be precisely manipulated by genetic modification. Here, we show that the transmembrane beta- barrel of an engineered alphaHL pore contains 3 recognition sites that can be used to identify all 4 DNA bases in an immobilized single-stranded DNA molecule, whether they are located in an otherwise homopolymeric DNA strand or in a heteropolymeric strand. The additional steps required to enable nanopore DNA sequencing are outlined.}, added-at = {2009-05-19T18:00:18.000+0200}, author = {Stoddart, David and Heron, Andrew J J. and Mikhailova, Ellina and Maglia, Giovanni and Bayley, Hagan}, biburl = {https://www.bibsonomy.org/bibtex/29f7135f00ee6690429661732b1fe9654/earthfare}, citeulike-article-id = {4381133}, description = {CiteULike: Everyone's library}, doi = {10.1073/pnas.0901054106}, interhash = {30c7e7c686820cd3b1dce241841916b9}, intrahash = {9f7135f00ee6690429661732b1fe9654}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {imported}, month = {April}, posted-at = {2009-05-19 15:35:52}, priority = {2}, timestamp = {2009-05-19T18:03:27.000+0200}, title = {Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore.}, url = {http://dx.doi.org/10.1073/pnas.0901054106}, year = 2009 }