%0 Journal Article %1 Harbeck2006 %A Harbeck, M. C. %A Chepurny, O. %A Nikolaev, V. O. %A Lohse, M. J. %A Holz, G. G. %A Roe, M. W. %D 2006 %J Sci STKE %K *Biosensing AMP/*analysis Animals Calcium/*analysis Cations, Cell Cyclic Cytosol/*chemistry Divalent Dyes Energy Exchange Factors/genetics Fluorescence Fluorescent Fura-2 Guanine Line, Messenger Mice Nucleotide Proteins/biosynthesis Recombinant Reproducibility Resonance Results Second Systems Techniques Transfection Transfer/methods Tumor of %N 353 %P pl6 %T Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16985238 %V 2006 %X Understanding the temporal and spatial integration of the Ca2+ and adenosine 3',5'-monophosphate (cAMP) signaling pathways requires concurrent measurements of both second messengers. Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging method allows investigation of the dynamic organization and integration of multiple levels of signal processing in single living cells.

@article{Harbeck2006, abstract = {Understanding the temporal and spatial integration of the Ca2+ and adenosine 3',5'-monophosphate (cAMP) signaling pathways requires concurrent measurements of both second messengers. Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging method allows investigation of the dynamic organization and integration of multiple levels of signal processing in single living cells.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Harbeck, M. C. and Chepurny, O. and Nikolaev, V. O. and Lohse, M. J. and Holz, G. G. and Roe, M. W.}, biburl = {https://www.bibsonomy.org/bibtex/234d34fcee09c764b4069c57dbe0c40dc/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {3a54ac0b3629dbea20e4aad2c22288fd}, intrahash = {34d34fcee09c764b4069c57dbe0c40dc}, issn = {1525-8882 (Electronic) 1525-8882 (Linking)}, journal = {Sci STKE}, keywords = {*Biosensing AMP/*analysis Animals Calcium/*analysis Cations, Cell Cyclic Cytosol/*chemistry Divalent Dyes Energy Exchange Factors/genetics Fluorescence Fluorescent Fura-2 Guanine Line, Messenger Mice Nucleotide Proteins/biosynthesis Recombinant Reproducibility Resonance Results Second Systems Techniques Transfection Transfer/methods Tumor of}, month = {Sep 19}, note = {Harbeck, Mark C Chepurny, Oleg Nikolaev, Viacheslav O Lohse, Martin J Holz, George G Roe, Michael W United States Science's STKE : signal transduction knowledge environment Sci STKE. 2006 Sep 19;2006(353):pl6.}, number = 353, pages = {pl6}, shorttitle = {Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells}, timestamp = {2010-12-14T18:12:13.000+0100}, title = {Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16985238}, volume = 2006, year = 2006 }