%0 Journal Article %1 Zou_2004_523 %A Zou, Hui %A Lifshitz, Lawrence M %A Tuft, Richard A %A Fogarty, Kevin E %A Singer, Joshua J %D 2004 %J Cell Calcium %K 15110142 Aniline Animals, Bufo Caffeine, Calcium Calcium, Cell Channel Channels, Compounds, Conductivity, Cytosol, Dyes, Electric Fluorescence, Fluorescent Gating, Gov't, In Ion Membr, Microscopy, Muscle, Myocytes, P.H.S., Patch-Clamp Research Smooth Support, Techniques, U.S. Vitro, Xanthenes, ane, marinus, %N 6 %P 523--533 %R 10.1016/j.ceca.2004.01.019 %T Imaging calcium entering the cytosol through a single opening of plasma membrane ion channels: SCCaFTs--fundamental calcium events. %U http://dx.doi.org/10.1016/j.ceca.2004.01.019 %V 35 %X Recently, it has become possible to record the localized fluorescence transient associated with the opening of a single plasma membrane Ca$^2+$ permeable ion channel using Ca$^2+$ indicators like fluo-3. These Single Channel Ca$^2+$ Fluorescence Transients (SCCaFTs) share some of the characteristics of such elementary events as Ca$^2+$ sparks and Ca$^2+$ puffs caused by Ca$^2+$ release from intracellular stores (due to the opening of ryanodine receptors and IP(3) receptors, respectively). In contrast to intracellular Ca$^2+$ release events, SCCaFTs can be observed while simultaneously recording the unitary channel currents using patch-clamp techniques to verify the channel openings. Imaging SCCaFTs provides a way to examine localized Ca$^2+$ handling in the vicinity of a channel with a known Ca$^2+$ influx, to obtain the Ca$^2+$ current passing through plasma membrane cation channels in near physiological solutions, to localize Ca$^2+$ permeable ion channels on the plasma membrane, and to estimate the Ca$^2+$ currents underlying those elementary events where the Ca$^2+$ currents cannot be recorded. Here we review studies of these fluorescence transients associated with caffeine-activated channels, L-type Ca$^2+$ channels, and stretch-activated channels. For the L-type Ca$^2+$ channel, SCCaFTs have been termed sparklets. In addition, we discuss how SCCaFTs have been used to estimate Ca$^2+$ currents using the rate of rise of the fluorescence transient as well as the signal mass associated with the total fluorescence increase.

@article{Zou_2004_523, abstract = {Recently, it has become possible to record the localized fluorescence transient associated with the opening of a single plasma membrane {C}a$^{2+}$ permeable ion channel using {C}a$^{2+}$ indicators like fluo-3. These Single Channel {C}a$^{2+}$ Fluorescence Transients (SCCaFTs) share some of the characteristics of such elementary events as {C}a$^{2+}$ sparks and {C}a$^{2+}$ puffs caused by {C}a$^{2+}$ release from intracellular stores (due to the opening of ryanodine receptors and IP(3) receptors, respectively). In contrast to intracellular {C}a$^{2+}$ release events, SCCaFTs can be observed while simultaneously recording the unitary channel currents using patch-clamp techniques to verify the channel openings. Imaging SCCaFTs provides a way to examine localized {C}a$^{2+}$ handling in the vicinity of a channel with a known {C}a$^{2+}$ influx, to obtain the {C}a$^{2+}$ current passing through plasma membrane cation channels in near physiological solutions, to localize {C}a$^{2+}$ permeable ion channels on the plasma membrane, and to estimate the {C}a$^{2+}$ currents underlying those elementary events where the {C}a$^{2+}$ currents cannot be recorded. Here we review studies of these fluorescence transients associated with caffeine-activated channels, L-type {C}a$^{2+}$ channels, and stretch-activated channels. For the L-type {C}a$^{2+}$ channel, SCCaFTs have been termed sparklets. In addition, we discuss how SCCaFTs have been used to estimate {C}a$^{2+}$ currents using the rate of rise of the fluorescence transient as well as the signal mass associated with the total fluorescence increase.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Zou, Hui and Lifshitz, Lawrence M and Tuft, Richard A and Fogarty, Kevin E and Singer, Joshua J}, biburl = {https://www.bibsonomy.org/bibtex/2c9032438a398ad3ef5ace916df6d75a9/hake}, description = {The whole bibliography file I use.}, doi = {10.1016/j.ceca.2004.01.019}, file = {Zou_2004_523.pdf:Zou_2004_523.pdf:PDF}, interhash = {3b2aabddecd3e6dbd158a439231d23c7}, intrahash = {c9032438a398ad3ef5ace916df6d75a9}, journal = {Cell Calcium}, key = 261, keywords = {15110142 Aniline Animals, Bufo Caffeine, Calcium Calcium, Cell Channel Channels, Compounds, Conductivity, Cytosol, Dyes, Electric Fluorescence, Fluorescent Gating, Gov't, In Ion Membr, Microscopy, Muscle, Myocytes, P.H.S., Patch-Clamp Research Smooth Support, Techniques, U.S. Vitro, Xanthenes, ane, marinus,}, month = Jun, number = 6, pages = {523--533}, pii = {S0143416004000387}, pmid = {15110142}, timestamp = {2009-06-03T11:21:39.000+0200}, title = {Imaging calcium entering the cytosol through a single opening of plasma membrane ion channels: {SCC}a{FT}s--fundamental calcium events.}, url = {http://dx.doi.org/10.1016/j.ceca.2004.01.019}, volume = 35, year = 2004 }