%0 Journal Article %1 citeulike:671172 %A Lu, Huihui %A Conneely, Grainne %A Crowe, Mark A. %A Aherne, Margaret %A Pravda, Miloslav %A Guilbault, George G. %D 2006 %J Analytica Chimica Acta %K amperometry competition immunoassay electrochemistry elisa %N 1 %P 116--123 %R 10.1016/j.aca.2006.03.108 %T Screening for testosterone, methyltestosterone, 19-nortestosterone residues and their metabolites in bovine urine with enzyme-linked immunosorbent assay (ELISA) %U http://www.sciencedirect.com/science/article/B6TF4-4JRT343-2/2/8e4e3b036586a0fced6188601412a7ec %V 570 %X This work reports on the development of rapid enzyme-linked immunosorbent assay for testosterone, methyltestosterone, 19-nortestosterone and their metabolites in real samples of bovine urine. The assays were based on the competition between an immobilised testosterone-BSA conjugate and the analyte for corresponding antibodies, followed by the use of secondary anti-species (alpha-IgG-HRP) to determine the degree of competition. Urine samples were analysed after 10-fold dilution with the buffer, omitting extraction and hydrolysis. The limits of detection calculated for the original sample were ca. 74, 266 and 131 pg ml-1 (ppt) for testosterone, methyltestosterone and 19-nortestosterone, respectively. In particular, testosterone and methyltestosterone assays offer the advantage to pick up both parent compounds and their major metabolites due to the high cross-reactivity pattern with corresponding antibody used in each assay. The concentration in bovine urine detected by developed method does indicate 19-nortestosterone administration to heifers. The developed assays were applied to urine samples of heifers treated with above androgens.

@article{citeulike:671172, abstract = {This work reports on the development of rapid enzyme-linked immunosorbent assay for testosterone, methyltestosterone, 19-nortestosterone and their metabolites in real samples of bovine urine. The assays were based on the competition between an immobilised testosterone-BSA conjugate and the analyte for corresponding antibodies, followed by the use of secondary anti-species ([alpha]-IgG-HRP) to determine the degree of competition. Urine samples were analysed after 10-fold dilution with the buffer, omitting extraction and hydrolysis. The limits of detection calculated for the original sample were ca. 74, 266 and 131 pg ml-1 (ppt) for testosterone, methyltestosterone and 19-nortestosterone, respectively. In particular, testosterone and methyltestosterone assays offer the advantage to pick up both parent compounds and their major metabolites due to the high cross-reactivity pattern with corresponding antibody used in each assay. The concentration in bovine urine detected by developed method does indicate 19-nortestosterone administration to heifers. The developed assays were applied to urine samples of heifers treated with above androgens.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Lu, Huihui and Conneely, Grainne and Crowe, Mark A. and Aherne, Margaret and Pravda, Miloslav and Guilbault, George G.}, biburl = {https://www.bibsonomy.org/bibtex/2c0bed494a1b0939f4a9a1f9a28b4f5d1/biblio24}, citeulike-article-id = {671172}, doi = {10.1016/j.aca.2006.03.108}, interhash = {443f445ba28adce6de3fd4d9828f73e3}, intrahash = {c0bed494a1b0939f4a9a1f9a28b4f5d1}, journal = {Analytica Chimica Acta}, keywords = {amperometry competition immunoassay electrochemistry elisa}, month = {June}, number = 1, pages = {116--123}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Screening for testosterone, methyltestosterone, 19-nortestosterone residues and their metabolites in bovine urine with enzyme-linked immunosorbent assay (ELISA)}, url = {http://www.sciencedirect.com/science/article/B6TF4-4JRT343-2/2/8e4e3b036586a0fced6188601412a7ec}, volume = 570, year = 2006 }