%0 Journal Article %1 citeulike:467036 %A Pashkov, V. N. %A Tsurupa, G. P. %A Griko, N. B. %A Skopinskaya, S. N. %A Yarkov, S. P. %C Branch of the M. M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region, Russia. %D 1992 %J Anal Biochem %K l-i-l-a ab_detection complement immunoassay homogeneous ag_detection %N 2 %P 341--347 %T The use of streptavidin-biotin interaction for preparation of reagents for complement-dependent liposome immunoassay of proteins: detection of latrotoxin. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1481990 %V 207 %X We have developed liposome sensitization by a protein, latrotoxin (LT), using immobilization of biotinylated LT via streptavidin with biotinylated phosphatidylethanolamine contained in liposomes. The use of such liposomes in the complement-dependent homogeneous liposome immune lysis assay (LILA) has allowed us to detect in the test sample as little as 2 micrograms/ml of polyclonal and 50-100 ng/ml of monoclonal IgG and IgM antibodies to LT. LT concentration in solution was determined by inhibition of immune lysis by free LT. The sensitivity of the LT assay varied from 1 x 10(-9) to 5-50 x 10(-9) M when antiserum (polyclonal antibodies) and monoclonal antibodies to LT were correspondingly used. The results show that a streptavidin-biotin spacer can be used to immobilize protein antigens on liposomes for a subsequent application in LILA. The suggested technique greatly simplifies the sensitization procedure and extends the applicability of the LILA.

@article{citeulike:467036, abstract = {We have developed liposome sensitization by a protein, latrotoxin (LT), using immobilization of biotinylated LT via streptavidin with biotinylated phosphatidylethanolamine contained in liposomes. The use of such liposomes in the complement-dependent homogeneous liposome immune lysis assay (LILA) has allowed us to detect in the test sample as little as 2 micrograms/ml of polyclonal and 50-100 ng/ml of monoclonal IgG and IgM antibodies to LT. LT concentration in solution was determined by inhibition of immune lysis by free LT. The sensitivity of the LT assay varied from 1 x 10(-9) to 5-50 x 10(-9) M when antiserum (polyclonal antibodies) and monoclonal antibodies to LT were correspondingly used. The results show that a streptavidin-biotin spacer can be used to immobilize protein antigens on liposomes for a subsequent application in LILA. The suggested technique greatly simplifies the sensitization procedure and extends the applicability of the LILA.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Branch of the M. M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region, Russia.}, author = {Pashkov, V. N. and Tsurupa, G. P. and Griko, N. B. and Skopinskaya, S. N. and Yarkov, S. P.}, biburl = {https://www.bibsonomy.org/bibtex/2972618874e18aa062a89d05caebd8d1e/biblio24}, citeulike-article-id = {467036}, interhash = {47a4c10daa31c1d4e6405e74e842f108}, intrahash = {972618874e18aa062a89d05caebd8d1e}, issn = {0003-2697}, journal = {Anal Biochem}, keywords = {l-i-l-a ab_detection complement immunoassay homogeneous ag_detection}, month = {December}, number = 2, pages = {341--347}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {The use of streptavidin-biotin interaction for preparation of reagents for complement-dependent liposome immunoassay of proteins: detection of latrotoxin.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1481990}, volume = 207, year = 1992 }