%0 Journal Article %1 schena1995quantitative %A Schena, Mark %A Shalon, Dari %A Davis, Ronald W %A Brown, Patrick O %D 1995 %J Science %K background dna methods microarrays %N 5235 %P 467-470 %R 10.1126/science.270.5235.467 %T Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray %U https://www.ncbi.nlm.nih.gov/pubmed/7569999 %V 270 %X A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

@article{schena1995quantitative, abstract = {A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.}, added-at = {2017-12-03T17:23:28.000+0100}, author = {Schena, Mark and Shalon, Dari and Davis, Ronald W and Brown, Patrick O}, biburl = {https://www.bibsonomy.org/bibtex/2c283c94f6f99e52328132e7cbc8a3463/artheibault}, description = {This article describes one of the first microarray experiments. For this experiment, researchers generated microarrays using the genome of Arabidopsis, a relatively simple model organism. The researchers admit that scaling up microarrays to study more complex genomes might be difficult. Still, the article gives a good overview of how microarrays work.}, doi = {10.1126/science.270.5235.467}, interhash = {4b38536684106504ef9b090927f11a79}, intrahash = {c283c94f6f99e52328132e7cbc8a3463}, journal = {Science}, keywords = {background dna methods microarrays}, number = 5235, pages = {467-470}, timestamp = {2017-12-03T17:23:28.000+0100}, title = {Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray}, url = {https://www.ncbi.nlm.nih.gov/pubmed/7569999}, volume = 270, year = 1995 }