%0 Journal Article %1 zhang2009inhibitory %A Zhang, Xuemei %A Song, Yu %A Xiong, Huanzhang %A Ci, Xinxin %A Li, Hongyu %A Yu, Lu %A Zhang, Lei %A Deng, Xuming %D 2009 %J International Immunopharmacology %K inflammation ivermectin %N 3 %P 354 - 359 %R https://doi.org/10.1016/j.intimp.2008.12.016 %T Inhibitory effects of ivermectin on nitric oxide and prostaglandin E2 production in LPS-stimulated RAW 264.7 macrophages %U http://www.sciencedirect.com/science/article/pii/S1567576909000034 %V 9 %X We have previously shown that ivermectin inhibits LPS-induced production of inflammatory cytokines. In the present study, we investigated the effect of ivermectin on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 macrophages. Ivermectin inhibited LPS-induced NO and PGE2 production. Consistent with these observations, the protein and mRNA expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) enzymes were inhibited by ivermectin in a concentration-dependent manner. Furthermore, the phosphorylation of p38, ERK1/2, and JNK in LPS-stimulated RAW 264.7 cells was suppressed by ivermectin in a dose-dependent manner. These results suggest that ivermectin suppresses NO and PGE2 production, as well as iNOS and COX-2 expression, by inhibiting phosphorylation of mitogen-activated protein kinases (MAPK) (p38, ERK1/2, and JNK) in LPS-stimulated RAW 264.7 cells.

@article{zhang2009inhibitory, abstract = {We have previously shown that ivermectin inhibits LPS-induced production of inflammatory cytokines. In the present study, we investigated the effect of ivermectin on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 macrophages. Ivermectin inhibited LPS-induced NO and PGE2 production. Consistent with these observations, the protein and mRNA expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) enzymes were inhibited by ivermectin in a concentration-dependent manner. Furthermore, the phosphorylation of p38, ERK1/2, and JNK in LPS-stimulated RAW 264.7 cells was suppressed by ivermectin in a dose-dependent manner. These results suggest that ivermectin suppresses NO and PGE2 production, as well as iNOS and COX-2 expression, by inhibiting phosphorylation of mitogen-activated protein kinases (MAPK) (p38, ERK1/2, and JNK) in LPS-stimulated RAW 264.7 cells.}, added-at = {2021-02-01T11:28:08.000+0100}, author = {Zhang, Xuemei and Song, Yu and Xiong, Huanzhang and Ci, Xinxin and Li, Hongyu and Yu, Lu and Zhang, Lei and Deng, Xuming}, biburl = {https://www.bibsonomy.org/bibtex/22a331b9a9a35e9e6621d0ddfff301445/fordham1}, description = {Inhibitory effects of ivermectin on nitric oxide and prostaglandin E2 production in LPS-stimulated RAW 264.7 macrophages - ScienceDirect}, doi = {https://doi.org/10.1016/j.intimp.2008.12.016}, interhash = {4c5f7bacebfc5cdd5063b14e8932bae2}, intrahash = {2a331b9a9a35e9e6621d0ddfff301445}, issn = {1567-5769}, journal = {International Immunopharmacology}, keywords = {inflammation ivermectin}, note = {paywalled}, number = 3, pages = {354 - 359}, timestamp = {2021-02-01T12:12:16.000+0100}, title = {Inhibitory effects of ivermectin on nitric oxide and prostaglandin E2 production in LPS-stimulated RAW 264.7 macrophages}, url = {http://www.sciencedirect.com/science/article/pii/S1567576909000034}, volume = 9, year = 2009 }