%0 Journal Article %1 jenkins2018comprehensive %A Jenkins, Timothy %A Formenti, Fabio %A Castro, Cecilia %A Piubelli, Chiara %A Perandin, Francesca %A Buonfrate, Dora %A Otranto, Domenica %A Griffin, Julian L %A Krause, Lutz %A Bisoffi, Zena %A Cantacessi, Cinzia %D 2018 %J Scientific Reports %K MS gastroenterology microbiome neurodegenerative %T A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area %U https://www.nature.com/articles/s41598-018-33937-3 %X Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas with multi-helminth infections and/or in volunteers with underlying gut disorders. Therefore, in this study, we explore the impact of natural mono-infections by the human parasite Strongyloides stercoralis on the faecal microbiota and metabolic profiles of a cohort of human volunteers from a non-endemic area of northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S−). Analyses of bacterial 16S rRNA high-throughput sequencing data revealed increased microbial alpha diversity and decreased beta diversity in the faecal microbial profiles of S+ subjects compared to S−. Furthermore, significant differences in the abundance of several bacterial taxa were observed between samples from S+ and S− subjects, and between S+ samples collected pre- and post-anthelmintic treatment. Faecal metabolite analysis detected marked increases in the abundance of selected amino acids in S+ subjects, and of short chain fatty acids in S− subjects. Overall, our work adds valuable knowledge to current understanding of parasite-microbiota associations and will assist future mechanistic studies aimed to unravel the causality of these relationships.

@article{jenkins2018comprehensive, abstract = {Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas with multi-helminth infections and/or in volunteers with underlying gut disorders. Therefore, in this study, we explore the impact of natural mono-infections by the human parasite Strongyloides stercoralis on the faecal microbiota and metabolic profiles of a cohort of human volunteers from a non-endemic area of northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S−). Analyses of bacterial 16S rRNA high-throughput sequencing data revealed increased microbial alpha diversity and decreased beta diversity in the faecal microbial profiles of S+ subjects compared to S−. Furthermore, significant differences in the abundance of several bacterial taxa were observed between samples from S+ and S− subjects, and between S+ samples collected pre- and post-anthelmintic treatment. Faecal metabolite analysis detected marked increases in the abundance of selected amino acids in S+ subjects, and of short chain fatty acids in S− subjects. Overall, our work adds valuable knowledge to current understanding of parasite-microbiota associations and will assist future mechanistic studies aimed to unravel the causality of these relationships.}, added-at = {2021-04-01T09:49:17.000+0200}, author = {Jenkins, Timothy and Formenti, Fabio and Castro, Cecilia and Piubelli, Chiara and Perandin, Francesca and Buonfrate, Dora and Otranto, Domenica and Griffin, Julian L and Krause, Lutz and Bisoffi, Zena and Cantacessi, Cinzia}, biburl = {https://www.bibsonomy.org/bibtex/283b297bcccbe7d1e2be8b13488bae77e/bellao}, interhash = {4cfbd5f073aa9c04cecd3cafe8882bf9}, intrahash = {83b297bcccbe7d1e2be8b13488bae77e}, journal = {Scientific Reports }, keywords = {MS gastroenterology microbiome neurodegenerative}, month = oct, timestamp = {2022-04-24T21:27:52.000+0200}, title = {A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area}, url = {https://www.nature.com/articles/s41598-018-33937-3}, year = {2018 } }