%0 Journal Article %1 WOS:000452048600002 %A Khan, Latif U %A Petry, Romana %A Paula, Amauri J %A Knobel, Marcelo %A Martinez, Diego Stefani T %C POSTFACH 101161, 69451 WEINHEIM, GERMANY %D 2018 %I WILEY-V C H VERLAG GMBH %J CHEMNANOMAT %K corona} europium; magnetite; nanoprobe; protein {bifunctional %N 12 %P 1202-1208 %R 10.1002/cnma.201800358 %T Protein Corona Formation on Magnetic Nanoparticles Conjugated with Luminescent Europium Complexes %V 4 %X The evaluation of the nanoparticles interactions with blood plasma enables a first and important insight on the organization of the adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles in human plasma. The difference in the surface chemistry of F3O4 functionalized with calixarene (+30 mV zeta-potential) and europium (III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV zeta-potential) affected the colloidal stability and hard corona composition of the nanoparticles, which were monitored by SDS-PAGE gel electrophoresis, differential centrifugal sedimentation analyses and luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA complex, the magnetic nanoparticles show lower adsorption affinity to proteins at higher blood plasma concentration. Moreover, the Eu3+ compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to bifunctional nanoparticles that were used as probes to evaluate the protein corona formation.

@article{WOS:000452048600002, abstract = {The evaluation of the nanoparticles interactions with blood plasma enables a first and important insight on the organization of the adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles in human plasma. The difference in the surface chemistry of F3O4 functionalized with calixarene (+30 mV zeta-potential) and europium (III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV zeta-potential) affected the colloidal stability and hard corona composition of the nanoparticles, which were monitored by SDS-PAGE gel electrophoresis, differential centrifugal sedimentation analyses and luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA complex, the magnetic nanoparticles show lower adsorption affinity to proteins at higher blood plasma concentration. Moreover, the Eu3+ compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to bifunctional nanoparticles that were used as probes to evaluate the protein corona formation.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {POSTFACH 101161, 69451 WEINHEIM, GERMANY}, author = {Khan, Latif U and Petry, Romana and Paula, Amauri J and Knobel, Marcelo and Martinez, Diego Stefani T}, biburl = {https://www.bibsonomy.org/bibtex/247180e1b84867a96cbf221b00c8590c0/ppgfis_ufc_br}, doi = {10.1002/cnma.201800358}, interhash = {5627877a4d768dec3523316ed63c8f1e}, intrahash = {47180e1b84867a96cbf221b00c8590c0}, issn = {2199-692X}, journal = {CHEMNANOMAT}, keywords = {corona} europium; magnetite; nanoprobe; protein {bifunctional}, number = 12, pages = {1202-1208}, publisher = {WILEY-V C H VERLAG GMBH}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Protein Corona Formation on Magnetic Nanoparticles Conjugated with Luminescent Europium Complexes}, tppubtype = {article}, volume = 4, year = 2018 }