%0 Journal Article %1 Banach1993 %A Banach, K. %A Bunemann, M. %A Huser, J. %A Pott, L. %D 1993 %J Pflugers Arch %K *Blood AMP/pharmacology Acetylcholine/pharmacology Adenylate Animals Atrial Atropine/pharmacology Bordetella/pharmacology Calcium Channels/*physiology Conductivity Cultured Cyclase Cyclic Electric Factors, Function Guinea Heart/*physiology Isoproterenol/pharmacology Pertussis Pigs Toxin Virulence beta/*physiology Receptor Cell Adrenergic %N 3-4 %P 245-50 %T Serum contains a potent factor that decreases beta-adrenergic receptor-stimulated L-type Ca2+ current in cardiac myocytes %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8391681 %V 423 %X L-type Ca2+ current (ICa) was measured in cultured atrial myocytes from hearts of adult guinea-pigs using whole-cell voltage clamp. Potentiation of ICa induced by beta-adrenergic stimulation (isoprenaline 2.10(-7) M) could be completely antagonized by diluted sera (1:100 v/v). Half-maximal inhibition of beta-receptor-stimulated ICa occurred at about 1:1000. Basal ICa was not affected by serum. Atropine in a concentration (10(-6) M) that completely antagonized the anti-adrenergic effect of acetylcholine (ACh, 2.10(-6) M) did not interfere with the effect of serum. In cells dialysed with cyclic adenosine monophosphate (cAMP)-containing (10(-4) M) pipette solution, potentiated ICa was insensitive to both ACh and serum. Preincubation of the myocytes with pertussis toxin almost completely abolished the anti-adrenergic effects of both ACh and serum. The potency of serum was not reduced by dialysis. It is concluded that serum contains a factor which, like ACh, inhibits beta-receptor-stimulated adenylyl cyclase via Gi-protein.

@article{Banach1993, abstract = {L-type Ca2+ current (ICa) was measured in cultured atrial myocytes from hearts of adult guinea-pigs using whole-cell voltage clamp. Potentiation of ICa induced by beta-adrenergic stimulation (isoprenaline 2.10(-7) M) could be completely antagonized by diluted sera (1:100 v/v). Half-maximal inhibition of beta-receptor-stimulated ICa occurred at about 1:1000. Basal ICa was not affected by serum. Atropine in a concentration (10(-6) M) that completely antagonized the anti-adrenergic effect of acetylcholine (ACh, 2.10(-6) M) did not interfere with the effect of serum. In cells dialysed with cyclic adenosine monophosphate (cAMP)-containing (10(-4) M) pipette solution, potentiated ICa was insensitive to both ACh and serum. Preincubation of the myocytes with pertussis toxin almost completely abolished the anti-adrenergic effects of both ACh and serum. The potency of serum was not reduced by dialysis. It is concluded that serum contains a factor which, like ACh, inhibits beta-receptor-stimulated adenylyl cyclase via Gi-protein.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Banach, K. and Bunemann, M. and Huser, J. and Pott, L.}, biburl = {https://www.bibsonomy.org/bibtex/2de6c759223bc2d884c5872690a889243/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {59b7eb94ecd56a672aa64d43974d375a}, intrahash = {de6c759223bc2d884c5872690a889243}, issn = {0031-6768 (Print) 0031-6768 (Linking)}, journal = {Pflugers Arch}, keywords = {*Blood AMP/pharmacology Acetylcholine/pharmacology Adenylate Animals Atrial Atropine/pharmacology Bordetella/pharmacology Calcium Channels/*physiology Conductivity Cultured Cyclase Cyclic Electric Factors, Function Guinea Heart/*physiology Isoproterenol/pharmacology Pertussis Pigs Toxin Virulence beta/*physiology Receptor Cell Adrenergic}, month = May, note = {Banach, K Bunemann, M Huser, J Pott, L Research Support, Non-U.S. Gov't Germany Pflugers Archiv : European journal of physiology Pflugers Arch. 1993 May;423(3-4):245-50.}, number = {3-4}, pages = {245-50}, shorttitle = {Serum contains a potent factor that decreases beta-adrenergic receptor-stimulated L-type Ca2+ current in cardiac myocytes}, timestamp = {2010-12-14T18:22:38.000+0100}, title = {Serum contains a potent factor that decreases beta-adrenergic receptor-stimulated L-type Ca2+ current in cardiac myocytes}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8391681}, volume = 423, year = 1993 }