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Digital holographic microscopy: a noninvasive contrastimaging technique allowing quantitative visualization ofliving cells with subwavelength axial accuracy

, , , , , , и .
Opt. Lett., 30 (5): 468--470 (марта 2005)
DOI: 10.1364/OL.30.000468

Аннотация

We have developed a digital holographic microscope (DHM), in a transmissionmode, especially dedicated to the quantitative visualization of phase objectssuch as living cells. The method is based on an original numerical algorithmpresented in detail elsewhere \Cuche et al.,Appl.Opt.38, 6994 (1999)\. DHM images of living cells in culture areshown for what is to our knowledge the first time. They represent the distributionof the optical path length over the cell, which has been measured with subwavelengthaccuracy. These DHM images are compared with those obtained by use of thewidely used phase contrast and Nomarski differential interference contrasttechniques.

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