%0 Journal Article %1 froschel2021plant %A Froschel, C. %A Komorek, J. %A Attard, A. %A Marsell, A. %A Lopez-Arboleda, W. A. %A Le Berre, J. %A Wolf, E. %A Geldner, N. %A Waller, F. %A Korte, A. %A Droge-Laser, W. %D 2021 %J Cell Host Microbe %K Arabidopsis/*microbiology/physiology myOwn uni_network %N 2 %P 299-310 e7 %R 10.1016/j.chom.2020.11.014 %T Plant roots employ cell-layer-specific programs to respond to pathogenic and beneficial microbes %U https://www.ncbi.nlm.nih.gov/pubmed/33378688 %V 29 %X Plant roots are built of concentric cell layers that are thought to respond to microbial infections by employing specific, genetically defined programs. Yet, the functional impact of this radial organization remains elusive, particularly due to the lack of genome-wide techniques for monitoring expression at a cell-layer resolution. Here, cell-type-specific expression of tagged ribosomes enabled the isolation of ribosome-bound mRNA to obtain cell-layer translatomes (TRAP-seq, translating ribosome affinity purification and RNA sequencing). After inoculation with the vascular pathogen Verticillium longisporum, pathogenic oomycete Phytophthora parasitica, or mutualistic endophyte Serendipita indica, root cell-layer responses reflected the fundamentally different colonization strategies of these microbes. Notably, V. longisporum specifically suppressed the endodermal barrier, which restricts fungal progression, allowing microbial access to the root central cylinder. Moreover, localized biosynthesis of antimicrobial compounds and ethylene differed in response to pathogens and mutualists. These examples highlight the power of this resource to gain insights into root-microbe interactions and to develop strategies in crop improvement.

@article{froschel2021plant, abstract = {Plant roots are built of concentric cell layers that are thought to respond to microbial infections by employing specific, genetically defined programs. Yet, the functional impact of this radial organization remains elusive, particularly due to the lack of genome-wide techniques for monitoring expression at a cell-layer resolution. Here, cell-type-specific expression of tagged ribosomes enabled the isolation of ribosome-bound mRNA to obtain cell-layer translatomes (TRAP-seq, translating ribosome affinity purification and RNA sequencing). After inoculation with the vascular pathogen Verticillium longisporum, pathogenic oomycete Phytophthora parasitica, or mutualistic endophyte Serendipita indica, root cell-layer responses reflected the fundamentally different colonization strategies of these microbes. Notably, V. longisporum specifically suppressed the endodermal barrier, which restricts fungal progression, allowing microbial access to the root central cylinder. Moreover, localized biosynthesis of antimicrobial compounds and ethylene differed in response to pathogens and mutualists. These examples highlight the power of this resource to gain insights into root-microbe interactions and to develop strategies in crop improvement.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Froschel, C. and Komorek, J. and Attard, A. and Marsell, A. and Lopez-Arboleda, W. A. and Le Berre, J. and Wolf, E. and Geldner, N. and Waller, F. and Korte, A. and Droge-Laser, W.}, biburl = {https://www.bibsonomy.org/bibtex/278d0e6bd7ce982f706e5b4018c9b9115/jvsi_all}, doi = {10.1016/j.chom.2020.11.014}, interhash = {739719955932c3278f028855044629bf}, intrahash = {78d0e6bd7ce982f706e5b4018c9b9115}, issn = {1934-6069 (Electronic) 1931-3128 (Linking)}, journal = {Cell Host Microbe}, keywords = {Arabidopsis/*microbiology/physiology myOwn uni_network}, note = {Froschel, Christian Komorek, Jaqueline Attard, Agnes Marsell, Alexander Lopez-Arboleda, William A Le Berre, Joelle Wolf, Elmar Geldner, Niko Waller, Frank Korte, Arthur Droge-Laser, Wolfgang eng 2020/12/31 Cell Host Microbe. 2021 Feb 10;29(2):299-310.e7. doi: 10.1016/j.chom.2020.11.014. Epub 2020 Dec 29.}, number = 2, pages = {299-310 e7}, timestamp = {2024-02-15T15:11:55.000+0100}, title = {Plant roots employ cell-layer-specific programs to respond to pathogenic and beneficial microbes}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/33378688}, volume = 29, year = 2021 }