%0 Journal Article %1 arima2007effect %A Arima, Yusuke %A Iwata, Hiroo %D 2007 %J Biomaterials %K adhesion cell chemistry nextgen protein %N 20 %P 3074 - 3082 %R http://dx.doi.org/10.1016/j.biomaterials.2007.03.013 %T Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers %U http://www.sciencedirect.com/science/article/pii/S0142961207002323 %V 28 %X Self-assembled monolayers (SAMs) of alkanethiols, which can provide flat and chemically well-defined surfaces, were employed as model surfaces to understand cellular interaction with artificial materials. \SAMs\ presenting a wide range of wettabilities were prepared by mixing two kinds of alkanethiols carrying terminal methyl (CH3), hydroxyl (OH), carboxylic acid (COOH), or amino (NH2) groups. Adhesion behavior of human umbilical vein endothelial cells (HUVECs) and HeLa cells on these mixed \SAMs\ were examined. The number of adhered \HUVECs\ reached a maximum on CH3/OH mixed \SAMs\ with a water contact angle of 40°, while cell adhesion increased with decreasing water contact angle up to 60–70° and then leveled off on CH3/COOH and CH3/NH2 mixed SAMs. Numbers of adhered HeLa cells showed a maximum on CH3/OH and CH3/COOH mixed \SAMs\ with a water contact angle of 50°. These facts suggest that cell adhesion is mainly determined by surface wettability, but is also affected by the surface functional group, its surface density, and the kinds of cells. The effect of exchange of adsorbed proteins on cell adhesion was also examined. \HUVECs\ were cultured on the mixed \SAMs\ preadsorbed with albumin. Cell adhesion was effectively prohibited on hydrophobic \SAMs\ pretreated with albumin. Albumin strongly adsorbed and resisted replacement by cell adhesive proteins on hydrophobic SAMs. On the other hand, cells adhered to albumin-adsorbed hydrophilic SAMs. Displacement of preadsorbed albumin with cell adhesive proteins effectively occurs on these hydrophilic SAMs. This effect contributes to induce \SAMs\ with moderate wettability to give suitable surfaces for cell adhesion.

@article{arima2007effect, abstract = {Self-assembled monolayers (SAMs) of alkanethiols, which can provide flat and chemically well-defined surfaces, were employed as model surfaces to understand cellular interaction with artificial materials. \{SAMs\} presenting a wide range of wettabilities were prepared by mixing two kinds of alkanethiols carrying terminal methyl (CH3), hydroxyl (OH), carboxylic acid (COOH), or amino (NH2) groups. Adhesion behavior of human umbilical vein endothelial cells (HUVECs) and HeLa cells on these mixed \{SAMs\} were examined. The number of adhered \{HUVECs\} reached a maximum on CH3/OH mixed \{SAMs\} with a water contact angle of 40°, while cell adhesion increased with decreasing water contact angle up to 60–70° and then leveled off on CH3/COOH and CH3/NH2 mixed SAMs. Numbers of adhered HeLa cells showed a maximum on CH3/OH and CH3/COOH mixed \{SAMs\} with a water contact angle of 50°. These facts suggest that cell adhesion is mainly determined by surface wettability, but is also affected by the surface functional group, its surface density, and the kinds of cells. The effect of exchange of adsorbed proteins on cell adhesion was also examined. \{HUVECs\} were cultured on the mixed \{SAMs\} preadsorbed with albumin. Cell adhesion was effectively prohibited on hydrophobic \{SAMs\} pretreated with albumin. Albumin strongly adsorbed and resisted replacement by cell adhesive proteins on hydrophobic SAMs. On the other hand, cells adhered to albumin-adsorbed hydrophilic SAMs. Displacement of preadsorbed albumin with cell adhesive proteins effectively occurs on these hydrophilic SAMs. This effect contributes to induce \{SAMs\} with moderate wettability to give suitable surfaces for cell adhesion. }, added-at = {2016-11-21T10:23:49.000+0100}, author = {Arima, Yusuke and Iwata, Hiroo}, biburl = {https://www.bibsonomy.org/bibtex/26c40b1605daadfda8d8eeafaf0fd7fb8/bkoch}, description = {Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers}, doi = {http://dx.doi.org/10.1016/j.biomaterials.2007.03.013}, interhash = {74c581c1ea199d496c83b49942580d7d}, intrahash = {6c40b1605daadfda8d8eeafaf0fd7fb8}, issn = {0142-9612}, journal = {Biomaterials }, keywords = {adhesion cell chemistry nextgen protein}, number = 20, pages = {3074 - 3082}, timestamp = {2016-11-21T10:23:49.000+0100}, title = {Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers }, url = {http://www.sciencedirect.com/science/article/pii/S0142961207002323}, volume = 28, year = 2007 }